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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-2-20
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pubmed:abstractText |
Dendritic cell (DC)-based immunotherapy has been hampered by the lack of suitable methods for antigen delivery. Here, we use biodegradable poly(D,L-lactide-co-glycolide) microspheres (PLGA-MS) as carriers of peptides and proteins for antigen delivery to human monocyte-derived DC (MoDC). Compared to soluble proteins, MHC classes I and II-restricted presentation of PLGA-MS-encapsulated proteins and peptides by MoDC was markedly prolonged and proteins were presented 50-fold more efficiently on class I molecules. The vaccination of mice with DC loaded with PLGA-MS-encapsulated proteins raised strong and persisting cytotoxic T cell responses. In conclusion, antigen delivery via PLGA-MS markedly enhanced the duration of antigen presentation by human MoDC and the potency of DC-based vaccination.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Delayed-Action Preparations,
http://linkedlifedata.com/resource/pubmed/chemical/Lactic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetanus Toxoid,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/influenza matrix peptide (58-66),
http://linkedlifedata.com/resource/pubmed/chemical/polylactic acid-polyglycolic acid...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-410X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1847-57
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16288821-Animals,
pubmed-meshheading:16288821-Antigen Presentation,
pubmed-meshheading:16288821-Cell Line,
pubmed-meshheading:16288821-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:16288821-Delayed-Action Preparations,
pubmed-meshheading:16288821-Dendritic Cells,
pubmed-meshheading:16288821-Humans,
pubmed-meshheading:16288821-Lactic Acid,
pubmed-meshheading:16288821-Mice,
pubmed-meshheading:16288821-Mice, Inbred C57BL,
pubmed-meshheading:16288821-Microspheres,
pubmed-meshheading:16288821-Peptide Fragments,
pubmed-meshheading:16288821-Peptides,
pubmed-meshheading:16288821-Polyglycolic Acid,
pubmed-meshheading:16288821-Polymers,
pubmed-meshheading:16288821-Proteins,
pubmed-meshheading:16288821-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:16288821-Tetanus Toxoid,
pubmed-meshheading:16288821-Vaccination,
pubmed-meshheading:16288821-Viral Matrix Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Encapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cells.
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pubmed:affiliation |
Research Department, Cantonal Hospital St. Gallen, CH-9007 St. Gallen, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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