Linked Life Data

16284195

Source:http://linkedlifedata.com/resource/pubmed/id/16284195

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2005-11-14
pubmed:abstractText
During early zebrafish (Danio rerio) development zygotic transcription does not begin until the mid-blastula transition (MBT) 3 h after fertilization. MBT demarcates transition from synchronous short cell cycles of S and M phases exclusively to full cycles encompassing G1 and G2 phases. Transcriptional profiling and RT-PCR analyses during these phases enabled us to determine that this shift corresponds to decreased transcript levels of S/M phase cell cycle control genes (e.g. ccna2, ccnb1, ccnb2 and ccne) and increased transcript levels of ccnd1, encoding cyclin D1, and orthologs of p21 (p21-like) and retinoblastoma (Rb-like 1). To investigate the regulation of this process further, the translation of ccnd1 mRNA, a G1/S checkpoint control element, was impaired by microinjection of ccnd1-specific morpholino phosphorodiamidate (MO) 20mer or hydroxyprolyl-phosphono peptide nucleic acid (HypNA-pPNA) 16mer antisense oligonucleotides. The resulting downregulation of cyclin D1 protein resulted in microophthalmia and microcephaly, but not lethality. The phenotypes were not seen with 3-mismatch MO 20mers or 1-mismatch HypNA-pPNA 16mers, and were rescued by an exogenous ccnd1 mRNA construct with five mismatches. Collectively, these results indicate that transcription of key molecular determinants of asynchronous cell cycle control in zebrafish embryos commences at MBT and that the reduction of cyclin D1 expression compromises zebrafish eye and head development.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-10068998, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-11017081, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-14565235, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-14579379, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-15187251, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-15545086, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-2482421, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-7557388, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-7664341, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-8547308, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-8589427, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-9001205, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-9421517, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-9479827, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-9793642, http://linkedlifedata.com/resource/pubmed/commentcorrection/16284195-9843981
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4914-21
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural