Source:http://linkedlifedata.com/resource/pubmed/id/16278432
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 12
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| pubmed:dateCreated |
2005-11-9
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| pubmed:abstractText |
A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0022-2615
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
54
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1183-7
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| pubmed:meshHeading |
pubmed-meshheading:16278432-Bacterial Proteins,
pubmed-meshheading:16278432-Base Sequence,
pubmed-meshheading:16278432-DNA Primers,
pubmed-meshheading:16278432-Escherichia coli,
pubmed-meshheading:16278432-Humans,
pubmed-meshheading:16278432-Klebsiella pneumoniae,
pubmed-meshheading:16278432-Polymerase Chain Reaction,
pubmed-meshheading:16278432-beta-Lactamases
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| pubmed:year |
2005
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| pubmed:articleTitle |
Rapid and simple detection of blaCTX-M genes by multiplex PCR assay.
|
| pubmed:affiliation |
Health Protection Agency West Midlands Public Health Laboratory, Birmingham Heartlands & Solihull NHS Trust, Birmingham B9 5SS, UK. li.xu@heartofengland.nhs.uk
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| pubmed:publicationType |
Journal Article
|