Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-12-5
pubmed:abstractText
Cytomegalovirus (CMV) is a major cause of congenital brain disease, and its neuropathogenesis may be related to viral infection of rapidly dividing, susceptible neural precursor cells (NPCs). In the present study, we evaluated the susceptibility of human fetal brain-derived NPCs (nestin(+), A2B5(+), CD133(+)) to infection with CMV. Data derived from these studies demonstrated that undifferentiated NPCs supported productive viral replication. After differentiation in the presence of serum, a treatment that promotes development of an astroglial cell phenotype (GFAP(+), nestin(-), A2B5(-)), viral expression was retained. However, differentiation of NPCs in medium containing platelet-derived growth factor and brain-derived neurotropic factor, conditions that support the development of neurons (Tuj-1(+), nestin(-), A2B5(-)), resulted in reduced viral expression, with corresponding decreased CMV major immediate-early promoter (MIEP) activity relative to undifferentiated cells. Further experiments showed that cellular differentiation into a neuronal phenotype was associated with elevated levels of various CCAAT/enhancer binding protein beta (C/EBP)-beta isoforms, which suppressed MIEP activity in cotransfected NPCs. Taken together, these data demonstrate that the susceptibility of primary human NPCs to CMV is retained concomitantly with differentiation into glial cells but is actively repressed following differentiation into neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/AC133 antigen, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Brain-Derived Neurotrophic Factor, http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein, http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances, http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Peptides, http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts, http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles, http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase, http://linkedlifedata.com/resource/pubmed/chemical/nestin, http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
839-50
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16273540-Antigens, CD, pubmed-meshheading:16273540-Blotting, Western, pubmed-meshheading:16273540-Brain, pubmed-meshheading:16273540-Brain-Derived Neurotrophic Factor, pubmed-meshheading:16273540-Cell Differentiation, pubmed-meshheading:16273540-Cell Proliferation, pubmed-meshheading:16273540-Cytomegalovirus, pubmed-meshheading:16273540-Fetus, pubmed-meshheading:16273540-Flow Cytometry, pubmed-meshheading:16273540-Gene Expression Regulation, pubmed-meshheading:16273540-Glial Fibrillary Acidic Protein, pubmed-meshheading:16273540-Glycoproteins, pubmed-meshheading:16273540-Growth Substances, pubmed-meshheading:16273540-Humans, pubmed-meshheading:16273540-Immunohistochemistry, pubmed-meshheading:16273540-Intermediate Filament Proteins, pubmed-meshheading:16273540-L-Lactate Dehydrogenase, pubmed-meshheading:16273540-Nerve Tissue Proteins, pubmed-meshheading:16273540-Neuroglia, pubmed-meshheading:16273540-Neurons, pubmed-meshheading:16273540-Peptides, pubmed-meshheading:16273540-Platelet-Derived Growth Factor, pubmed-meshheading:16273540-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16273540-Stem Cells, pubmed-meshheading:16273540-Tetrazolium Salts, pubmed-meshheading:16273540-Thiazoles, pubmed-meshheading:16273540-Time Factors, pubmed-meshheading:16273540-Transfection, pubmed-meshheading:16273540-beta-Galactosidase
pubmed:year
2005
pubmed:articleTitle
Neural precursor cell susceptibility to human cytomegalovirus diverges along glial or neuronal differentiation pathways.
pubmed:affiliation
Neuroimmunology Laboratory, Center for Infectious Diseases and Microbiology Translational Research, University of Minnesota Medical School, Minneapolis, 55455, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural