|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
22
|
|
pubmed:dateCreated |
2005-10-28
|
|
pubmed:abstractText |
A search for dominant-negative mutant polypeptides hampering infectious bursal disease virus (IBDV) replication has been undertaken. We have found that expression of a mutant version of the VP3 structural polypeptide known as VP3/M3, partially lacking the domain responsible for the interaction with the virus-encoded RNA polymerase, efficiently interferes with the IBDV replication cycle. Transformed cells stably expressing VP3/M3 show a significant reduction (up to 96%) in their ability to support IBDV growth. Our findings provide a new tool for the characterization of the IBDV replication cycle and might facilitate the generation of genetically modified chicken lines with a reduced susceptibility to IBDV infection.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-10400796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-10717289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-11114190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-11118356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-12388690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-12551984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-12664296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-12743301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-15192698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-8385328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-8637876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16254378-9311897
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
79
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
14437-41
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Intracellular interference of infectious bursal disease virus.
|
|
pubmed:affiliation |
Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, Madrid, Spain. jfrodrig@cnb.uam.es.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
