Source:http://linkedlifedata.com/resource/pubmed/id/16232725
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-10-19
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| pubmed:abstractText |
The effects of zeolites and monocations on the protein synthesis in a cell-free system derived from wheat germ were investigated. M type of synthetic zeolite markedly enhanced the translation efficiency. Whereas this kind of stimulatory effect of zeolite in an Escherichia coli cell-free system resulted from a change in the salt compositions of the reaction solution with the addition of zeolite, the enhancement of protein synthesis in a wheat germ cell-free system was not due to the ion exchange reaction of zeolites. From the results of mRNA stability analysis, it was found that zeolite could stabilize the mRNA in a wheat germ cell-free protein synthesis system. The stabilization of mRNA by the simple addition of zeolites is useful for the enhancement of protein synthesis in a wheat germ cell-free system, since conventional methods to improve mRNA stability, such as the addition of nuclease inhibitor, have not been effective for a wheat germ cell-free system.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:issn |
1389-1723
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
89
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
193-5
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| pubmed:year |
2000
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| pubmed:articleTitle |
Stabilization effect of zeolite on DHFR mRNA in a wheat germ cell-free protein synthesis system.
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| pubmed:affiliation |
School of Chemical Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea.
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| pubmed:publicationType |
Journal Article
|