Linked Life Data

16227656

Source:http://linkedlifedata.com/resource/pubmed/id/16227656

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-10-17
pubmed:abstractText
Articular chondrocytes are exposed to significant changes in extracellular osmolarity during normal joint activity, which can lead to changes in cell volume and metabolism of the extracellular matrix (ECM). Chondrocytes can respond to cell swelling/shrinking by volume regulatory pathways, but the signalling pathways are poorly understood although a role for the cytoskeleton is frequently implicated. Here, we have investigated the effects of disruption of the chondrocyte F-actin cytoskeleton on the recovery of cell volume by RVD. The cytoskeleton was perturbed using the relatively specific agent latrunculin B (5 microM; 30 min) and loss of F-actin integrity quantified using fluorescent phalloidin-labelling and confocal laser scanning microscopy (CLSM). Imaging of isolated chondrocytes labelled with Fura-2 to measure the fluorescence associated with cell volume changes, showed that the extent of hypo-osmotic swelling was unaffected by latrunculin B treatment. Two categories of the chondrocyte RVD response were observed: 'fast' RVD where at 3 min post-osmotic challenge there was a recovery in cell fluorescence of >or=80%, whereas other cells exhibited 'slow' RVD. Latrunculin B increased the proportion of chondrocytes demonstrating 'fast' RVD by approximately 10 fold and reduced those cells showing 'slow' RVD. An inhibitor of chondrocyte RVD (REV 5901) had no significant effect on the integrity of the cytoskeleton showing that the RVD response could be inhibited independent of the state of the F-actin cytoskeleton. These results suggest that the intact cortical F-actin cytoskeleton has a restraining effect on the RVD response of isolated bovine articular chondrocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0006-355X
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-93
pubmed:dateRevised
2007-8-13
pubmed:meshHeading
pubmed-meshheading:16227656-Actins, pubmed-meshheading:16227656-Animals, pubmed-meshheading:16227656-Bicyclo Compounds, Heterocyclic, pubmed-meshheading:16227656-Cartilage, Articular, pubmed-meshheading:16227656-Cattle, pubmed-meshheading:16227656-Cell Size, pubmed-meshheading:16227656-Cells, Cultured, pubmed-meshheading:16227656-Chondrocytes, pubmed-meshheading:16227656-Cytoskeleton, pubmed-meshheading:16227656-Lipoxygenase Inhibitors, pubmed-meshheading:16227656-Mechanotransduction, Cellular, pubmed-meshheading:16227656-Microscopy, Confocal, pubmed-meshheading:16227656-Microscopy, Fluorescence, pubmed-meshheading:16227656-Osmosis, pubmed-meshheading:16227656-Quinolines, pubmed-meshheading:16227656-Stimulation, Chemical, pubmed-meshheading:16227656-Thiazoles, pubmed-meshheading:16227656-Thiazolidines
pubmed:year
2005
pubmed:articleTitle
Stimulation of regulatory volume decrease (RVD) by isolated bovine articular chondrocytes following F-actin disruption using latrunculin B.
pubmed:affiliation
School of Biomedical and Clinical Laboratory Science, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't