Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-1-25
pubmed:abstractText
Recent advances have increased the purity of hematopoietic stem cells (HSCs) isolated from young mouse bone marrow. However, little attention has been paid to the purity of HSCs from other contexts. Although Thy-1 low Sca-1+ Lineage- c-kit+ cells from young bone marrow are highly enriched for HSCs (1 in 5 cells gives long-term multilineage reconstitution after transplantation into irradiated mice), the same population from old, reconstituted, or cytokine-mobilized mice engrafts much less efficiently (1 in 78 to 1 in 185 cells gives long-term multilineage reconstitution). To test whether we could increase the purity of HSCs isolated from these contexts, we examined the SLAM family markers CD150 and CD48. All detectable HSCs from old, reconstituted, and cyclophosphamide/G-CSF-mobilized mice were CD150+ CD48-, just as in normal young bone marrow. Thy-1 low Sca-1+ Lineage- c-kit+ cells from old, reconstituted, or mobilized mice included mainly CD48+ and/or CD150- cells that lacked reconstituting ability. CD150+ CD48- Sca-1+ Lineage- c-kit+ cells from old, reconstituted, or mobilized mice were much more highly enriched for HSCs, with 1 in 3 to 1 in 7 cells giving long-term multilineage reconstitution. SLAM family receptor expression is conserved among HSCs from diverse contexts, and HSCs from old, reconstituted, and mobilized mice engraft relatively efficiently after transplantation when contaminating cells are eliminated.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-10688785, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-11067876, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-11724967, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-11823053, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-12215650, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-12393529, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-1346154, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-14563317, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-14662343, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-14738758, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-14738767, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-14744992, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-15260986, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-15459755, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-15827136, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-15831702, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-15989959, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-1672767, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-7479772, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-7541305, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-7849289, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-8662508, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-8689561, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-8704206, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-8782459, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-9050878, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-9057635, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-9160664, http://linkedlifedata.com/resource/pubmed/commentcorrection/16219798-9169840
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1, http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Alkylating, http://linkedlifedata.com/resource/pubmed/chemical/CD150 antigen, http://linkedlifedata.com/resource/pubmed/chemical/CD48 antigen, http://linkedlifedata.com/resource/pubmed/chemical/Cyclophosphamide, http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating..., http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins, http://linkedlifedata.com/resource/pubmed/chemical/Ly6a protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
924-30
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16219798-Aging, pubmed-meshheading:16219798-Animals, pubmed-meshheading:16219798-Antigens, CD, pubmed-meshheading:16219798-Antigens, Ly, pubmed-meshheading:16219798-Antigens, Thy-1, pubmed-meshheading:16219798-Antineoplastic Agents, Alkylating, pubmed-meshheading:16219798-Cell Separation, pubmed-meshheading:16219798-Cyclophosphamide, pubmed-meshheading:16219798-Glycoproteins, pubmed-meshheading:16219798-Graft Survival, pubmed-meshheading:16219798-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:16219798-Hematopoietic Stem Cell Mobilization, pubmed-meshheading:16219798-Hematopoietic Stem Cell Transplantation, pubmed-meshheading:16219798-Hematopoietic Stem Cells, pubmed-meshheading:16219798-Immunoglobulins, pubmed-meshheading:16219798-Membrane Proteins, pubmed-meshheading:16219798-Mice, pubmed-meshheading:16219798-Proto-Oncogene Proteins c-kit, pubmed-meshheading:16219798-Receptors, Cell Surface, pubmed-meshheading:16219798-Whole-Body Irradiation
pubmed:year
2006
pubmed:articleTitle
SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.
pubmed:affiliation
Howard Hughes Medical Institute, Department of Internal Medicine, Ann Arbor, MI, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.
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