Linked Life Data

16219684

Source:http://linkedlifedata.com/resource/pubmed/id/16219684

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 20
pubmed:dateCreated
2005-10-12
pubmed:abstractText
Although the small Arf-like GTPases Arl1-3 are highly conserved eukaryotic proteins, they remain relatively poorly characterized. The yeast and mammalian Arl1 proteins bind to the Golgi complex, where they recruit specific structural proteins such as Golgins. Yeast Arl1p directly interacts with Mon2p/Ysl2p, a protein that displays some sequence homology to the large Sec7 guanine exchange factors (GEFs) of Arf1. Mon2p also binds the putative aminophospholipid translocase (APT) Neo1p, which performs essential function(s) in membrane trafficking. Our detailed analysis reveals that Mon2p contains six distinct amino acid regions (A to F) that are conserved in several other uncharacterized homologs in higher eukaryotes. As the conserved A, E and F domains are unique to these homologues, they represent the signature of a new protein family. To investigate the role of these domains, we made a series of N- and C-terminal deletions of Mon2p. Although fluorescence and biochemical studies showed that the B and C domains (also present in the large Sec7 GEFs) predominantly mediate interaction with Golgi/endosomal membranes, growth complementation studies revealed that the C-terminal F domain is essential for the activity of Mon2p, indicating that Mon2p might also function independently of Arl1p. We provide evidence that Mon2p is required for efficient recycling from endosomes to the late Golgi. Intriguingly, although transport of CPY to the vacuole was nearly normal in the Deltamon2 strain, we found the constitutive delivery of Aminopeptidase 1 from the cytosol to the vacuole to be almost completely blocked. Finally, we show that Mon2p exhibits genetic and physical interactions with Dop1p, a protein with a putative function in cell polarity. We propose that Mon2p is a scaffold protein with novel conserved domains, and is involved in multiple aspects of endomembrane trafficking.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4751-64
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16219684-Aminopeptidases, pubmed-meshheading:16219684-Biological Transport, pubmed-meshheading:16219684-Centrifugation, Density Gradient, pubmed-meshheading:16219684-Conserved Sequence, pubmed-meshheading:16219684-Cytoplasm, pubmed-meshheading:16219684-Endosomes, pubmed-meshheading:16219684-Golgi Apparatus, pubmed-meshheading:16219684-Guanine Nucleotide Exchange Factors, pubmed-meshheading:16219684-Homeostasis, pubmed-meshheading:16219684-Intracellular Membranes, pubmed-meshheading:16219684-Membrane Proteins, pubmed-meshheading:16219684-Multiprotein Complexes, pubmed-meshheading:16219684-Protein Binding, pubmed-meshheading:16219684-Recombinant Fusion Proteins, pubmed-meshheading:16219684-Saccharomyces cerevisiae, pubmed-meshheading:16219684-Saccharomyces cerevisiae Proteins, pubmed-meshheading:16219684-Sequence Homology, pubmed-meshheading:16219684-Vacuoles, pubmed-meshheading:16219684-Vesicular Transport Proteins
pubmed:year
2005
pubmed:articleTitle
Yeast Mon2p is a highly conserved protein that functions in the cytoplasm-to-vacuole transport pathway and is required for Golgi homeostasis.
pubmed:affiliation
Division of Biology, Department of Cellular and Molecular Medicine, and the Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093-0668, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural