Source:http://linkedlifedata.com/resource/pubmed/id/16219372
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2005-12-2
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pubmed:abstractText |
We have cloned, expressed and characterized a alpha-actinin-like protein of Entamoeba histolytica. Analysis of the primary structure reveals that the essential domains of the alpha-actinin protein family are conserved: an N-terminus actin-binding domain, a C-terminus calcium-binding domain and a central helical rod domain. However, the rod domain of this Entamoeba protein is considerably shorter than the rod domain in alpha-actinins of higher organisms. The cloned Entamoeba 63 kDa protein is recognized by conventional alpha-actinin antibodies as well as binds and cross-links filamentous actin and calcium ions in the same manner as alpha-actinins. Despite the shorter rod domain this protein has conserved the most important functions of alpha-actinins. Therefore, it is suggested that this 63 kDa protein is an atypical and ancestral alpha-actinin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0166-6851
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16219372-Actinin,
pubmed-meshheading:16219372-Actins,
pubmed-meshheading:16219372-Animals,
pubmed-meshheading:16219372-Calcium,
pubmed-meshheading:16219372-Circular Dichroism,
pubmed-meshheading:16219372-Cloning, Molecular,
pubmed-meshheading:16219372-Entamoeba histolytica,
pubmed-meshheading:16219372-Microscopy, Electron, Transmission,
pubmed-meshheading:16219372-Sequence Analysis, DNA,
pubmed-meshheading:16219372-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2006
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pubmed:articleTitle |
Characterization of Entamoeba histolytica alpha-actinin.
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pubmed:affiliation |
Biochemistry, Umeå University, SE-901 87 Umeå, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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