Linked Life Data

16205164

Source:http://linkedlifedata.com/resource/pubmed/id/16205164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2005-10-5
pubmed:abstractText
The joint capsule is a key component in posttraumatic joint contractures. The capsule is described as thickened, but little data exist supporting the observation. Our hypotheses were that mRNA levels of (1) collagen; (2) decorin and biglycan; (3) matrix metalloproteinases; and (4) tissue inhibitors of matrix metalloproteinases were significantly elevated in anterior joint capsules obtained from 11 patients having surgery for posttraumatic contractures when compared with nine elbows, from organ donors, that were free of contractures. Reverse transcription-polymerase chain reaction was used to evaluate mRNA expression normalized to a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase. In the joint capsules of the patients with elbow contractures, relative mRNA levels were increased for: collagen Types I, III, and V (1.5-2.5 times); biglycan (1.5 times); and matrix metalloproteinases-1, -2, -9, -13, and -15 (1.6-3.9 times). In contrast, expression of tissue inhibitors of matrix metalloproteinases-1, -2, and -4 were decreased (1/3-3/4 times) in the capsules of patients with contractures. There was no difference between the groups in relative mRNA expression for decorin, matrix metalloproteinases-8, -14 and -16, and tissue inhibitor of matrix metalloproteinase-3. The results indicate that joint capsule matrix molecule mRNA levels are altered in the chronic stages of posttraumatic elbow contractures in humans, potentially creating an environment with high matrix turnover rates.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-921X
pubmed:author
pubmed:issnType
Print
pubmed:volume
439
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
228-34
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed-meshheading:16205164-Adolescent, pubmed-meshheading:16205164-Adult, pubmed-meshheading:16205164-Biglycan, pubmed-meshheading:16205164-Chronic Disease, pubmed-meshheading:16205164-Collagen Type I, pubmed-meshheading:16205164-Collagen Type III, pubmed-meshheading:16205164-Collagen Type V, pubmed-meshheading:16205164-Contracture, pubmed-meshheading:16205164-Elbow Joint, pubmed-meshheading:16205164-Extracellular Matrix Proteins, pubmed-meshheading:16205164-Female, pubmed-meshheading:16205164-Gene Expression, pubmed-meshheading:16205164-Humans, pubmed-meshheading:16205164-Joint Capsule, pubmed-meshheading:16205164-Male, pubmed-meshheading:16205164-Matrix Metalloproteinases, pubmed-meshheading:16205164-Middle Aged, pubmed-meshheading:16205164-Proteoglycans, pubmed-meshheading:16205164-RNA, Messenger, pubmed-meshheading:16205164-Tissue Inhibitor of Metalloproteinases
pubmed:year
2005
pubmed:articleTitle
High rate of joint capsule matrix turnover in chronic human elbow contractures.
pubmed:affiliation
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Calgary, Canada. hildebrk@ucalgary.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't