Linked Life Data

16193381

Source:http://linkedlifedata.com/resource/pubmed/id/16193381

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-9-29
pubmed:abstractText
Chemotherapy in glioma is poorly effective: the blood-brain barrier and intrinsic and/or acquired drug resistance of tumor cells could partly explain this lack of major effect. We investigated expression of P-glycoprotein (Pgp), multidrug resistance protein (MRP) 1, MRP3, MRP5 and glutathione-S-transferase pi (GST-pi) in malignant glioma patients. Cytofluorimetric analysis of 48 glioma specimens and 21 primary cultures showed high levels of MRP1, moderate levels of MRP5 and low levels of Pgp, GST-pi and MRP3. Immunohistochemistry (25 glioma specimens) showed expression of GST-pi (66.7% of cases), MRP1 (51.3%), MRP5 (45.8%), Pgp (34.8%) and MRP3 (29.9%) in tumor cells. Moreover, analysis of tumor samples by real time quantitative PCR showed mRNA expression of all investigated genes. Tumor vasculature, analyzed in glioma specimens and in tumor derived endothelial cells, showed expression of all investigated proteins. Non-tumor brain samples (from a patient with arteriovenous malformation and from one with epilepsy), normal human astrocytes and cultured endothelial cells were also analyzed: astrocytes and endothelial cells expressed the highest levels of the investigated proteins, mainly MRP1 and MRP5. No significant differences in proteins expression were detected between primary or recurrent gliomas, suggesting that glioma chemoresistance is mostly intrinsic. Therefore, we detected, for the first time, the presence of MRP3 and MRP5 on glioma specimens--both in tumor and endothelial cells--and we delineated an expression profile of chemoresistance proteins in glioma. The possible association of inhibitors of drug efflux pumps with chemotherapy could be investigated to improve drugs delivery into the tumor and their cytotoxic effects.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0167-594X
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16193381-Adult, pubmed-meshheading:16193381-Aged, pubmed-meshheading:16193381-Brain Neoplasms, pubmed-meshheading:16193381-Drug Resistance, Multiple, pubmed-meshheading:16193381-Drug Resistance, Neoplasm, pubmed-meshheading:16193381-Endothelium, Vascular, pubmed-meshheading:16193381-Female, pubmed-meshheading:16193381-Glioma, pubmed-meshheading:16193381-Glutathione S-Transferase pi, pubmed-meshheading:16193381-Humans, pubmed-meshheading:16193381-Immunoenzyme Techniques, pubmed-meshheading:16193381-Male, pubmed-meshheading:16193381-Microscopy, Fluorescence, pubmed-meshheading:16193381-Middle Aged, pubmed-meshheading:16193381-Multidrug Resistance-Associated Proteins, pubmed-meshheading:16193381-P-Glycoproteins, pubmed-meshheading:16193381-Polymerase Chain Reaction, pubmed-meshheading:16193381-RNA, Messenger, pubmed-meshheading:16193381-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2005
pubmed:articleTitle
Expression of drug resistance proteins Pgp, MRP1, MRP3, MRP5 and GST-pi in human glioma.
pubmed:affiliation
Istituto Nazionale Neurologico C. Besta, Via Celoria 11, 20133 Milan, Italy.
pubmed:publicationType
Journal Article, Comparative Study