|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0052128,
umls-concept:C0205263,
umls-concept:C0242606,
umls-concept:C0439097,
umls-concept:C0599946,
umls-concept:C0961954,
umls-concept:C1100939,
umls-concept:C1373059,
umls-concept:C1420590,
umls-concept:C1547348,
umls-concept:C1705241
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2005-12-6
|
|
pubmed:abstractText |
Previous reports have indicated that the use of delta agonists may prove to be a viable therapeutic tool as an analgesic agent without conventional opioid side effects. In addition, recent evidence suggests that delta ligands may exert neuroprotective effects under a variety of toxin insults. The aim of the present studies was to assess the ability of delta agonists (peptide: [D-Pen(2,5)] enkephalin (DPDPE), non-peptide: (+)-4-[(aR)-a-((2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-80)) and antagonists (naltrindole) to modify dichlorofluorescein (DCFH) fluorescence in the presence of the peroxynitrite generator, 3-morpholinylsydnoneimine chloride (SIN-1) or HIV-protein, TAT(1-72) (TAT) in SK-N-SH cells. Both DPDPE (100 nM) and SNC-80 (250 nM) attenuated (30-50%) the increased oxidative stress in the presence of SIN-1. This effect was partially reversed by addition of naltrindole, suggesting involvement of delta receptors. Peroxynitrite radicals are involved in neurotoxicity associated with TAT. Incubation with TAT (10-250 nM) demonstrated a concentration-dependent increase in oxidative stress up to 200% over control values. Preincubation with delta agonists reduced 50 nM TAT-mediated oxidative stress 15-40%, which was partially reversed by naltrindole. Increasing log-concentrations of DPDPE or SNC-80 (0.01-100 microM) attenuated TAT-mediated oxidative stress up to 50% at 100 microM. In conclusion, these data demonstrate that both peptide and non-peptide delta agonists can partially attenuate intracellular oxidative stress, in part through a receptor-mediated mechanism. This suggests that delta ligands may have therapeutic usefulness in HIV patients beyond analgesia.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-morpholino-sydnonimine,
http://linkedlifedata.com/resource/pubmed/chemical/4-(alpha-(4-allyl-2,5-dimethyl-1-pip...,
http://linkedlifedata.com/resource/pubmed/chemical/Benzamides,
http://linkedlifedata.com/resource/pubmed/chemical/Enkephalin, D-Penicillamine (2,5)-,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Molsidomine,
http://linkedlifedata.com/resource/pubmed/chemical/Naltrexone,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Opioid Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/diacetyldichlorofluorescein,
http://linkedlifedata.com/resource/pubmed/chemical/naltrindole,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...,
http://linkedlifedata.com/resource/pubmed/chemical/tat peptide (1-72), Human...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0161-813X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
27
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
101-7
|
|
pubmed:dateRevised |
2011-10-4
|
|
pubmed:meshHeading |
pubmed-meshheading:16168488-Benzamides,
pubmed-meshheading:16168488-Cell Line, Tumor,
pubmed-meshheading:16168488-Cell Membrane,
pubmed-meshheading:16168488-Dose-Response Relationship, Drug,
pubmed-meshheading:16168488-Enkephalin, D-Penicillamine (2,5)-,
pubmed-meshheading:16168488-Fluoresceins,
pubmed-meshheading:16168488-Free Radicals,
pubmed-meshheading:16168488-Gene Products, tat,
pubmed-meshheading:16168488-Humans,
pubmed-meshheading:16168488-Molsidomine,
pubmed-meshheading:16168488-Naltrexone,
pubmed-meshheading:16168488-Narcotic Antagonists,
pubmed-meshheading:16168488-Opioid Peptides,
pubmed-meshheading:16168488-Oxidative Stress,
pubmed-meshheading:16168488-Piperazines,
pubmed-meshheading:16168488-Receptors, Opioid, delta,
pubmed-meshheading:16168488-tat Gene Products, Human Immunodeficiency Virus
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Delta opioid agonists attenuate TAT(1-72)-induced oxidative stress in SK-N-SH cells.
|
|
pubmed:affiliation |
Oklahoma State University, Center for Health Sciences, Departments of Pharmacology, Physiology and Forensic Sciences, 1111 West 17th Street, Tulsa, OK 74107-1898, USA. walladr@chs.okstate.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
