Linked Life Data

16143635

Source:http://linkedlifedata.com/resource/pubmed/id/16143635

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-10-28
pubmed:abstractText
The uptake of nutrients is essential for the survival of bacterial cells. Many specialized systems have evolved, such as the maltose-dependent ABC transport system that transfers oligosaccharides through the cytoplasmic membrane. The maltose/maltodextrin-binding protein (MBP) serves as an initial high-affinity binding component in the periplasm that delivers the bound sugar into the cognate ABC transporter MalFGK(2). We have investigated the domain motions induced by the binding of the ligand maltotriose into the binding cleft using molecular dynamics simulations. We find that MBP is predominantly in the open state without ligand and in the closed state with ligand bound. Oligosaccharide binding induces a closure motion (30.0 degrees rotation), whereas ligand removal leads to domain opening (32.6 degrees rotation) around a well-defined hinge affecting key areas relevant for chemotaxis and transport. Our simulations suggest that a "hook-and-eye" motif is involved in the binding. A salt bridge between Glu-111 and Lys-15 forms that effectively locks the protein-ligand complex in a semiclosed conformation inhibiting any further opening and promoting complete closure. This previously unrecognized feature seems to secure the ligand in the binding site and keeps MBP in the closed conformation and suggests a role in the initial steps of substrate transport.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-10742172, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11287673, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11399072, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11501994, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11790091, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11806910, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-11959992, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12217701, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12463636, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12468528, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12475936, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12520056, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-1282354, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-12935905, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-1420181, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-14343300, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-14530390, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-15117946, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-15147839, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-1537797, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-1660187, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-2002054, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-3049536, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-3050132, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-3510191, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-383708, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-3894331, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-3945310, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-4215651, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-5938952, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-7521874, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-776623, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8108382, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8132630, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8411172, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8663400, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8861200, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8897598, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-8951381, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9309217, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9482874, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9489922, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9529892, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9571045, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9641984, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9927672, http://linkedlifedata.com/resource/pubmed/commentcorrection/16143635-9931266
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3362-71
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
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