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rdf:type |
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lifeskim:mentions |
umls-concept:C0009325,
umls-concept:C0033268,
umls-concept:C0059438,
umls-concept:C0071649,
umls-concept:C0449432,
umls-concept:C1150174,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1704263,
umls-concept:C1705248,
umls-concept:C2340138
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pubmed:issue |
4
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pubmed:dateCreated |
2005-9-5
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pubmed:abstractText |
Catechins such as epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), and epigallocatechin (EGC) are polyphenol components of green tea. EGCG is the major component and has been reported to possess a wide range of biological properties including anti-fibrogenic activity. In hepatic fibrosis, activated hepatic stellate cells (HSCs) play a central role. In this study, we investigated the effect of catechins, including EGCG, on collagen production and collagenase activity in rat primary HSCs and activated human HSC-derived TWNT-4 cells. EGCG (50 microM) suppressed type I collagen production in rat HSCs more than ECG (50 microM) did; however, EGC (50 microM) did not show suppressive effects. EGCG also inhibited both collagen production and collagenase activity (active matrix metalloproteinase-1 [MMP-1]) in a dose-dependent manner, but did not affect the tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) production in TWNT-4 cells. Real-time PCR unexpectedly revealed that EGCG enhanced the transcription of type I collagen and TIMP-1, but did not affect the transcription of alpha-smooth muscle actin (alpha-SMA), and reduced the transcription MMP-1 in TWNT-4 cells. These findings demonstrated that EGCG inhibited collagen production regardless of enhanced collagen transcription and suppressed collagenase activity, and suggested that EGCG might have therapeutic potential for liver fibrosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Catechin,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tea,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...,
http://linkedlifedata.com/resource/pubmed/chemical/epicatechin gallate,
http://linkedlifedata.com/resource/pubmed/chemical/epigallocatechin gallate,
http://linkedlifedata.com/resource/pubmed/chemical/gallocatechol
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1107-3756
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
677-81
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16142404-Actins,
pubmed-meshheading:16142404-Animals,
pubmed-meshheading:16142404-Antioxidants,
pubmed-meshheading:16142404-Catechin,
pubmed-meshheading:16142404-Cell Line,
pubmed-meshheading:16142404-Cells, Cultured,
pubmed-meshheading:16142404-Collagen,
pubmed-meshheading:16142404-Collagenases,
pubmed-meshheading:16142404-Dose-Response Relationship, Drug,
pubmed-meshheading:16142404-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16142404-Gene Expression,
pubmed-meshheading:16142404-Humans,
pubmed-meshheading:16142404-Liver,
pubmed-meshheading:16142404-Male,
pubmed-meshheading:16142404-Matrix Metalloproteinase 1,
pubmed-meshheading:16142404-Muscle, Smooth,
pubmed-meshheading:16142404-RNA, Messenger,
pubmed-meshheading:16142404-Rats,
pubmed-meshheading:16142404-Rats, Wistar,
pubmed-meshheading:16142404-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16142404-Tea,
pubmed-meshheading:16142404-Tissue Inhibitor of Metalloproteinase-1
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pubmed:year |
2005
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pubmed:articleTitle |
Epigallocatechin-3-gallate, a polyphenol component of green tea, suppresses both collagen production and collagenase activity in hepatic stellate cells.
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pubmed:affiliation |
Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. nakamuta@intmed3.med.kyushu-u.ac.jp
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pubmed:publicationType |
Journal Article,
Comparative Study
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