Source:http://linkedlifedata.com/resource/pubmed/id/16128923
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
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| pubmed:dateCreated |
2005-8-30
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| pubmed:abstractText |
The pigtail macaque (Macaca nemestrina) is a common model for the study of AIDS. The pigtail major histocompatibility complex class I allele Mane-A*10 restricts an immunodominant simian immunodeficiency virus (SIV) Gag epitope (KP9) which rapidly mutates to escape T cell recognition following acute simian/human immunodeficiency virus infection. Two technologies for the detection of Mane-A*10 in outbred pigtail macaques were developed: reference strand-mediated conformational analysis and sequence-specific primer polymerase chain reaction. A Mane-A*10/KP9 tetramer was then developed to quantify CD8(+) T lymphocytes primed by multigenic DNA vaccination, which have previously been difficult to detect using standard interferon-gamma-based T cell assays. We also demonstrate mutational escape at KP9 following acute SIV infection. Mane-A*10(+) animals have lower set point SIV levels than Mane-A*10(-) animals, suggesting a significant fitness cost of escape. These studies pave the way for a more robust understanding of HIV vaccines in pigtail macaques.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0047-2565
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| pubmed:author |
pubmed-author:BrooksAndrew GAG,
pubmed-author:ChungAmyA,
pubmed-author:DaleC JaneCJ,
pubmed-author:DavenportMiles PMP,
pubmed-author:De RoseRobertR,
pubmed-author:DeyD KDK,
pubmed-author:FernandezCaroline SCS,
pubmed-author:KentStephen JSJ,
pubmed-author:KrebsKendall CKC,
pubmed-author:O'ConnorDavid HDH,
pubmed-author:SmithMiranda ZMZ,
pubmed-author:WatkinsDavid IDI
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| pubmed:issnType |
Print
|
| pubmed:volume |
34
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
282-93
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:16128923-Alleles,
pubmed-meshheading:16128923-Animals,
pubmed-meshheading:16128923-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16128923-DNA Primers,
pubmed-meshheading:16128923-Evolution, Molecular,
pubmed-meshheading:16128923-Flow Cytometry,
pubmed-meshheading:16128923-Gene Products, gag,
pubmed-meshheading:16128923-Genes, MHC Class I,
pubmed-meshheading:16128923-Heteroduplex Analysis,
pubmed-meshheading:16128923-Immunodominant Epitopes,
pubmed-meshheading:16128923-Macaca nemestrina,
pubmed-meshheading:16128923-Monkey Diseases,
pubmed-meshheading:16128923-Mutation,
pubmed-meshheading:16128923-Polymerase Chain Reaction,
pubmed-meshheading:16128923-Simian Acquired Immunodeficiency Syndrome,
pubmed-meshheading:16128923-Simian immunodeficiency virus
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| pubmed:year |
2005
|
| pubmed:articleTitle |
The pigtail macaque MHC class I allele Mane-A*10 presents an immundominant SIV Gag epitope: identification, tetramer development and implications of immune escape and reversion.
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| pubmed:affiliation |
Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic., Australia.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
|