Linked Life Data

16122962

Source:http://linkedlifedata.com/resource/pubmed/id/16122962

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-11-1
pubmed:abstractText
Human erythrocyte cells contain specific, active insulin receptor. However, the physiological relevance of this receptor is unclear. Here we show that Ca2+ influx is 4-fold higher in erythrocytes upon insulin stimulation. These effects are dose-dependent and are diminished by insulin concentrations of 150 nM and higher. The insulin-stimulated Ca2+ influx depends on a tyrosine-kinase activity and involves the verapamil-dependent Ca2+ channels. Elevated intracellular Ca2+, in association with the Ca2+-binding protein, calmodulin, stimulates erythrocytes 6-phosphofructo-1-kinase activity. This activation involves the detachment of the enzyme from erythrocyte membranes, which has been described as an important mechanism of glycolysis regulation on these cells. Altogether, these results support evidence that insulin may increases glucose consumption in human erythrocytes, through a mechanism involving Ca2+ influx, calmodulin and the detachment of 6-phosphofructo-1-kinase from the erythrocyte membrane.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1096-7192
pubmed:author
pubmed:issnType
Print
pubmed:volume
86
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
392-400
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Calcium influx: a possible role for insulin modulation of intracellular distribution and activity of 6-phosphofructo-1-kinase in human erythrocytes.
pubmed:affiliation
Laboratório de Enzimologia e Controle do Metabolismo (LabECoM), Departamento de Fármacos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't