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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2005-11-15
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pubmed:abstractText |
Hydoxyurea induces senescence-like growth arrest in normal human fibroblasts. p21(WAF/CIP1/SDI1), a cyclin dependent kinase inhibitor, was found to be upregulated during this growth arrest. Levels of p21(WAF/CIP1/SDI1) protein and mRNA were increased nine-fold by hydroxyurea in these cells. In order to determine whether p21(WAF/CIP1/SDI1) mRNA is increased by hydroxyurea at the transcriptional level, human fibroblast cells were transfected with reporter constructs containing a p21(WAF/CIP1/SDI1) promoter fragment and then treated with hydroxyurea. The luciferase activities in the reporter-transfected fibroblast cells were not increased by hydroxyurea, indicating that p21(WAF/CIP1/SDI1) transcription was not elevated by hydroxyurea. The half-life of the p21(WAF/CIP1/SDI1) mRNA was increased by 2.5-fold but that of p21(WAF/CIP1/SDI1) protein was not. Our results suggest that increased mRNA stability is the major mechanism of p21(WAF/CIP1/SDI1) elevation in the hydroxyurea-induced growth arrest of human fibroblasts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0047-6374
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pubmed:author |
pubmed-author:KangTae-ChunTC,
pubmed-author:KimHyun-SeokHS,
pubmed-author:KimJaebongJ,
pubmed-author:KimJong-IlJI,
pubmed-author:KimMin-JuMJ,
pubmed-author:LeeHyun-YongHY,
pubmed-author:LeeJae-YongJY,
pubmed-author:OhSoo-JinSJ,
pubmed-author:ParkJae-BongJB,
pubmed-author:ParkJoo-InJI,
pubmed-author:ParkSang-ChulSC,
pubmed-author:ParkSeong-HoonSH,
pubmed-author:ShinJong-YeonJY,
pubmed-author:WonMoo-HoMH,
pubmed-author:YeoEui-juEJ
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pubmed:issnType |
Print
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1255-61
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16111738-Blotting, Northern,
pubmed-meshheading:16111738-Blotting, Western,
pubmed-meshheading:16111738-Cell Aging,
pubmed-meshheading:16111738-Cells, Cultured,
pubmed-meshheading:16111738-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:16111738-Dose-Response Relationship, Drug,
pubmed-meshheading:16111738-Fibroblasts,
pubmed-meshheading:16111738-Gene Expression Regulation,
pubmed-meshheading:16111738-Genes, Reporter,
pubmed-meshheading:16111738-Humans,
pubmed-meshheading:16111738-Hydroxyurea,
pubmed-meshheading:16111738-Luciferases,
pubmed-meshheading:16111738-Nucleic Acid Conformation,
pubmed-meshheading:16111738-Plasmids,
pubmed-meshheading:16111738-Promoter Regions, Genetic,
pubmed-meshheading:16111738-RNA, Messenger,
pubmed-meshheading:16111738-Time Factors,
pubmed-meshheading:16111738-Transcription, Genetic,
pubmed-meshheading:16111738-Transfection,
pubmed-meshheading:16111738-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
p21WAF/CIP1/SDI1 is upregulated due to increased mRNA stability during hydroxyurea-induced senescence of human fibroblasts.
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pubmed:affiliation |
Department of Biochemistry, College of Medicine, Hallym University, 1 Okchon-dong, Chuncheon, Gangwon-do 200-702, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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