Source:http://linkedlifedata.com/resource/pubmed/id/16110513
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-10-24
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| pubmed:abstractText |
Motor axons in the trunk of the developing zebrafish exit from the ventral spinal cord in one ventral root per hemisegment and grow on a common path toward the region of the horizontal myoseptum, where they select their specific pathways. Tenascin-C, a component of the extracellular matrix, is concentrated in this choice region. Adaxial cells and other myotomal cells express tenascin-C mRNA, suggesting that these cells are the source of tenascin-C protein. Overexpressing an axon repellent fragment containing the cysteine-rich region and the epidermal growth factor-like repeats of tenascin-C led to retarded growth of ventral motor nerves between their spinal exit point and the horizontal myoseptum. Injection of a protein fragment containing the same part of tenascin-C also induced slower growth of motor nerves. Conversely, knock down of tenascin-C protein resulted in abnormal lateral branching of ventral motor nerves. In the zebrafish unplugged mutant, in which axons display pathfinding defects in the region of the horizontal myoseptum, tenascin-C immunoreactivity was not detectable in this region, indicating an abnormal extracellular matrix in unplugged. We conclude that tenascin-C is part of a specialized extracellular matrix in the region of the horizontal myoseptum that influences the growth of motor axons.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tenascin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1058-8388
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| pubmed:author | |
| pubmed:copyrightInfo |
Developmental Dynamics 234:550-566, 2005. (c) 2005 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
234
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
550-66
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| pubmed:dateRevised |
2011-1-14
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| pubmed:meshHeading |
pubmed-meshheading:16110513-Amino Acid Sequence,
pubmed-meshheading:16110513-Animals,
pubmed-meshheading:16110513-Axons,
pubmed-meshheading:16110513-Cell Differentiation,
pubmed-meshheading:16110513-Cloning, Molecular,
pubmed-meshheading:16110513-Cysteine,
pubmed-meshheading:16110513-DNA, Complementary,
pubmed-meshheading:16110513-Epidermal Growth Factor,
pubmed-meshheading:16110513-Gene Expression Regulation, Developmental,
pubmed-meshheading:16110513-Humans,
pubmed-meshheading:16110513-Immunohistochemistry,
pubmed-meshheading:16110513-Molecular Sequence Data,
pubmed-meshheading:16110513-Motor Neurons,
pubmed-meshheading:16110513-Mutation,
pubmed-meshheading:16110513-RNA, Messenger,
pubmed-meshheading:16110513-Sequence Alignment,
pubmed-meshheading:16110513-Sequence Homology, Amino Acid,
pubmed-meshheading:16110513-Tenascin,
pubmed-meshheading:16110513-Zebrafish
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| pubmed:year |
2005
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| pubmed:articleTitle |
Tenascin-C is involved in motor axon outgrowth in the trunk of developing zebrafish.
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| pubmed:affiliation |
Zentrum für Molekulare Neurobiologie, Hamburg, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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