Source:http://linkedlifedata.com/resource/pubmed/id/16108093
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2005-8-17
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pubmed:abstractText |
We analyzed gene expression profiles of normal mouse fibroblast BALB/c 3T3 cells and its SV40 transformant SV-T2 cells using our originally developed cell surface marker DNA microarray, which is prepared on a diamond-like carbon-coated glass. As a result, CD62L and IL-6 receptor alpha gene expressions were upregulated in SV-T2 and were thought to be candidates for call surface markers of the cells. The result of microarray analysis was validated by real-time quantitative PCR immunohistochemistry and biological assays. These data show that our cell surface marker DNA microarray should be useful in finding the candidates of cell type-specific surface markers.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
334
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16108093-Animals,
pubmed-meshheading:16108093-BALB 3T3 Cells,
pubmed-meshheading:16108093-Biological Markers,
pubmed-meshheading:16108093-Cell Line,
pubmed-meshheading:16108093-Fibroblasts,
pubmed-meshheading:16108093-Gene Expression Profiling,
pubmed-meshheading:16108093-Membrane Proteins,
pubmed-meshheading:16108093-Mice,
pubmed-meshheading:16108093-Oligonucleotide Array Sequence Analysis
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pubmed:year |
2005
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pubmed:articleTitle |
Identification of cell surface marker candidates on SV-T2 cells using DNA microarray on DLC-coated glass.
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pubmed:affiliation |
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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