| pubmed-article:16083877 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0033578 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0242643 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C1844596 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0242640 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0030054 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0596902 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0205332 | lld:lifeskim |
| pubmed-article:16083877 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:16083877 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:16083877 | pubmed:dateCreated | 2005-8-24 | lld:pubmed |
| pubmed-article:16083877 | pubmed:abstractText | Expression of the multidrug resistance (MDR) transporter P-glycoprotein (P-gp) has been demonstrated to be regulated by hypoxia-inducible factor-1alpha (HIF-1alpha) and inhibited by intracellular reactive oxygen species (ROS). Herein, P-gp and HIF-1alpha expression were investigated in multicellular prostate tumor spheroids overexpressing the ROS-generating enzyme Nox-1 in comparison to the mother cell line DU-145. In Nox-1-overexpressing tumor spheroids (DU-145Nox1) generation of ROS as well as expression of Nox-1 was significantly increased as compared to DU-145 tumor spheroids. ROS generation was significantly inhibited in the presence of the NADPH-oxidase antagonists diphenylen-iodonium chloride (DPI) and 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF). Albeit growth kinetic of DU-145Nox1 tumor spheroids was decreased as compared to DU-145 spheroids, elevated expression of Ki-67 was observed indicating increased cell cycle activity. In DU-145Nox1 tumor spheroids, expression of HIF-1alpha as well as P-gp was significantly decreased as compared to DU-145 spheroids, which resulted in an increased retention of the anticancer agent doxorubicin. Pretreatment with the free radical scavengers vitamin E and vitamin C increased the expression of P-gp as well as HIF-1alpha in Nox-1-overexpressing cells, whereas no effect of free radical scavengers was observed on mdr-1 mRNA expression. In summary, the data of the present study demonstrate that the development of P-gp-mediated MDR is abolished under conditions of elevated ROS levels, suggesting that the MDR phenotype can be circumvented by modest increase of intracellular ROS generation. | lld:pubmed |
| pubmed-article:16083877 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16083877 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16083877 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:16083877 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16083877 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16083877 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16083877 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:PetrosJohnJ | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:HeschelerJürg... | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:SauerHeinrich... | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:WartenbergMar... | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:GrünheckFrank... | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:HoffmannEddaE | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:SchwindtHeinr... | lld:pubmed |
| pubmed-article:16083877 | pubmed:author | pubmed-author:ArnoldJ... | lld:pubmed |
| pubmed-article:16083877 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16083877 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:16083877 | pubmed:volume | 579 | lld:pubmed |
| pubmed-article:16083877 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16083877 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16083877 | pubmed:pagination | 4541-4549 | lld:pubmed |
| pubmed-article:16083877 | pubmed:dateRevised | 2006-6-27 | lld:pubmed |
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| pubmed-article:16083877 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16083877 | pubmed:articleTitle | Reactive oxygen species-linked regulation of the multidrug resistance transporter P-glycoprotein in Nox-1 overexpressing prostate tumor spheroids. | lld:pubmed |
| pubmed-article:16083877 | pubmed:affiliation | Department of Cell Biology, GKSS Research Center, Teltow, Germany. | lld:pubmed |
| pubmed-article:16083877 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:5243 | entrezgene:pubmed | pubmed-article:16083877 | lld:entrezgene |
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