Linked Life Data

16055679

Source:http://linkedlifedata.com/resource/pubmed/id/16055679

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-9-20
pubmed:abstractText
Vernalization, the requirement of a long exposure to low temperatures to accelerate flowering, is an essential adaptation of plants to cold winters. The vernalization gene VRN-1 plays an important role in this process in diploid (Triticum monococcum) and polyploid wheat (Triticum aestivum). We have recently shown that the diploid wheat VRN-A(m)1 gene was similar to the Arabidopsis (Arabidopsis thaliana L. Heynh.) APETALA1 meristem identity gene. We also showed that dominant Vrn-A(m)1 alleles were the result of loss-of-function mutations in regulatory regions recognized by a VRN-1 repressor, likely VRN-2. This model predicts that only the dominant Vrn-1 allele will be transcribed in lines carrying both recessive and dominant alleles. Here, we confirm this prediction in young isogenic lines of hexaploid wheat carrying different dominant Vrn-A1, Vrn-B1, and Vrn-D1 alleles, and also in heterozygous VRN-1 diploid wheat plants. However, a few weeks later, transcripts from the recessive alleles were also detected in both the polyploid and heterozygous diploid spring plants. Transcription of the recessive alleles was preceded by a reduction of the transcript levels of VRN-2. These results suggest that the dominant Vrn-1 allele or a gene regulated by VRN-1 down-regulates the VRN-2 repressor facilitating the transcription of the recessive alleles in unvernalized plants. We also show here that the level of VRN-1 transcripts in early developmental stages is critical for flowering initiation. A reduction of VRN-1 transcript levels by RNA interference delayed apex transition to the reproductive stage, increased the number of leaves, and delayed heading time by 2 to 3 weeks. We hypothesize that the coordinated transcription of dominant and recessive alleles may contribute to an earlier attainment of the VRN-1 transcript level threshold required to trigger flowering initiation in polyploid wheat.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-10912677, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-11197326, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-11719192, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-11846609, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-12114624, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-12582873, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-12730378, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-12805638, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-12913142, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-1359429, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-14557548, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-14593187, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-14712276, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-14712277, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-15016992, http://linkedlifedata.com/resource/pubmed/commentcorrection/16055679-15480533
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2364-73
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Regulation of VRN-1 vernalization genes in normal and transgenic polyploid wheat.
pubmed:affiliation
Department of Plant Sciences, University of California, Davis, California 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.