Source:http://linkedlifedata.com/resource/pubmed/id/16054259
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-11-8
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| pubmed:abstractText |
A total of 294 honey samples produced in Denmark, Norway and Sweden were studied for the presence of Clostridium botulinum types A, B, E and F by using a multiplex-PCR method. The samples consisted of honeycombs taken directly from beehives, and extracted honey representing several hives or apiaries. The prevalence of C. botulinum showed a significant variation between Denmark, Norway and Sweden, the proportions of positive samples being 26%, 10% and 2%, respectively. The major serotype detected was type B. When analysed with pulsed-field gel electrophoresis (PFGE) using restriction enzyme SacII, the 24 strains isolated produced eight different PFGE patterns. At a similarity level of 95%, four clusters were produced, three of which contained 20 of the 24 analysed strains. One of the clusters included isolates from both Denmark and Norway.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0168-1605
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
105
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
145-51
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16054259-Bacterial Typing Techniques,
pubmed-meshheading:16054259-Clostridium botulinum,
pubmed-meshheading:16054259-Clostridium botulinum type A,
pubmed-meshheading:16054259-Clostridium botulinum type B,
pubmed-meshheading:16054259-Clostridium botulinum type E,
pubmed-meshheading:16054259-Clostridium botulinum type F,
pubmed-meshheading:16054259-Cluster Analysis,
pubmed-meshheading:16054259-DNA, Bacterial,
pubmed-meshheading:16054259-Denmark,
pubmed-meshheading:16054259-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:16054259-Food Contamination,
pubmed-meshheading:16054259-Food Microbiology,
pubmed-meshheading:16054259-Honey,
pubmed-meshheading:16054259-Norway,
pubmed-meshheading:16054259-Phylogeny,
pubmed-meshheading:16054259-Polymerase Chain Reaction,
pubmed-meshheading:16054259-Prevalence,
pubmed-meshheading:16054259-Restriction Mapping,
pubmed-meshheading:16054259-Serotyping,
pubmed-meshheading:16054259-Sweden
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Prevalence and diversity of Clostridium botulinum types A, B, E and F in honey produced in the Nordic countries.
|
| pubmed:affiliation |
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 Helsinki, Finland. mari.nevas@helsinki.fi
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|