Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-7-27
pubmed:abstractText
The ppk gene encodes polyphosphate kinase (Ppk), an enzyme that catalyses the polymerization of inorganic phosphate into long chains of polyphosphate (polyP). An insertion mutation in ppk causes a decrease in adaptive mutation in Escherichia coli strain FC40. Adaptive mutation in FC40 mostly results from error-prone DNA polymerase IV (Pol IV), encoded by dinB; most of the antimutagenic phenotype of the ppk mutant disappears in a dinB mutant strain. In addition, the ppk mutant causes a decrease in growth-dependent mutations produced by overexpressing Pol IV. However, the amount of Pol IV protein is unchanged in the ppk mutant strain, indicating that the activity or fidelity of Pol IV is altered. Adaptive mutation is inhibited both by the absence of Ppk, which results in low amounts of polyP, and by overproduction of Ppk, which results in high amounts of polyP, suggesting that an optimal level of polyP is necessary. Taken together, these results suggest a novel mechanism involving polyP that directly or indirectly regulates DNA polymerase activity or fidelity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10217781, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10610800, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10801133, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10829079, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10871396, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10872445, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-10978509, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-11263491, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-11333217, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-11463382, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-11474114, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-11683261, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-12035760, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-12045089, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-12208995, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-12270817, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-12760017, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-14235589, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-14617178, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-14633999, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-14981237, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-15020458, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-1511865, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-15187082, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-15262914, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-15262917, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-15262920, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-1916241, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-2830458, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-344137, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-6095300, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-6771759, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7029544, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7040679, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7608087, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7716545, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7716546, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7768888, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7777535, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-7828809, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8023163, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8023164, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8146657, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8257106, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8380170, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8385889, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8655553, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8770582, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-8849879, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9045812, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9261133, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9326588, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9366549, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9383162, http://linkedlifedata.com/resource/pubmed/commentcorrection/16045619-9391106
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
751-61
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Polyphosphate kinase regulates error-prone replication by DNA polymerase IV in Escherichia coli.
pubmed:affiliation
Department of Biology, Indiana University, 1001 E. Third Street, Bloomington, IN 47405, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural