Source:http://linkedlifedata.com/resource/pubmed/id/16039398
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2005-7-25
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pubmed:abstractText |
Marijuana smoking is associated with inflammation, cellular atypia, and molecular dysregulation of the tracheobronchial epithelium. While marijuana smoke shares many components in common with tobacco, it also contains a high concentration of Delta9-tetrahydrocannabinol (THC). The potential contribution of THC to airway injury was assessed by exposing primary cultures of human small airway epithelial (SAE) cells to THC (0.1-10.0 microg/ml) for either 1 day or 7 days. THC induced a time- and concentration-dependent decrease in cell viability, ATP level, and mitochondrial membrane potential. Using a targeted gene expression array, we observed acute changes (24 h) in the expression of mRNA for caspase-8, catalase, Bax, early growth response-1, cytochrome P4501A1 (CYP1A1), metallothionein 1A, PLAB, and heat shock factor 1 (HSF1). After 7 days of exposure, decrease in expression of mRNA for heat shock proteins (HSPs) and the pro-apoptotic protein Bax was observed, while expression of GADD45A, IL-1A, CYP1A1, and PTGS-2 increased significantly. These findings suggest a contribution of THC to DNA damage, inflammation, and alterations in apoptosis. Treatment with selected prototypical toxicants, 2,3,7,8-tetrachlorodibenznzo-p-dioxin (TCDD) and carbonyl cyanide-p-(trifluoramethoxy)-phenyl hydrazone (FCCP), produced partially overlapping gene expression profiles suggesting some similarity in mechanism of action with THC. THC, delivered as a component of marijuana smoke, may induce a profile of gene expression that contributes to the pulmonary pathology associated with marijuana use.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0378-4274
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
158
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-107
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16039398-Adenosine Triphosphate,
pubmed-meshheading:16039398-Cell Survival,
pubmed-meshheading:16039398-Cells, Cultured,
pubmed-meshheading:16039398-Dose-Response Relationship, Drug,
pubmed-meshheading:16039398-Epithelial Cells,
pubmed-meshheading:16039398-Gene Expression,
pubmed-meshheading:16039398-Hallucinogens,
pubmed-meshheading:16039398-Humans,
pubmed-meshheading:16039398-Marijuana Abuse,
pubmed-meshheading:16039398-Membrane Potentials,
pubmed-meshheading:16039398-Mitochondria,
pubmed-meshheading:16039398-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16039398-RNA, Messenger,
pubmed-meshheading:16039398-Respiratory Mucosa,
pubmed-meshheading:16039398-Tetrahydrocannabinol
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pubmed:year |
2005
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pubmed:articleTitle |
Gene expression changes in human small airway epithelial cells exposed to Delta9-tetrahydrocannabinol.
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pubmed:affiliation |
Division of Pulmonary and Critical Care, Department of Medicine, 14-184 Warren Hall, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1690, USA. tsarafian@mednet.ucla.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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