Source:http://linkedlifedata.com/resource/pubmed/id/16036621
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4-5
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| pubmed:dateCreated |
2005-7-22
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| pubmed:abstractText |
Transcriptional regulation of the human alpha 2(I) procollagen proximal promoter involves the interaction of trans-acting factors at the inverted CCAAT box (G/CBE) located at position -80 and an adjacent GGAGGCCC-box at -70. Both these elements have previously been shown to be essential for activity of the human promoter. This study investigated nucleotide differences at three sites (-74, -72 and -71) between the human and mouse promoters that were sufficient to abolish trans-acting factor binding with the mouse sequence (GGAGACGT). Two distinct DNA-protein interactions were detected on the human -107/+54 promoter fragment while a single interaction was observed at the equivalent mouse promoter. One of these factors is the CCAAT-binding factor (CBF) and it's binding was observed on both the human and mouse promoters. Although the GGAGGCCC DNA-binding element was not detected on the mouse promoter, GGAGGCC-binding proteins were present in mouse nuclear extracts as observed by their interaction with the human promoter. Functional analysis of the human and mouse -343/+54 and -107/+54 promoter regions revealed significant differences between species; the human constructs having higher activity than the mouse. The differences in promoter activity between species may in part be a result of the nucleotide differences in the GGAGGCCC-box. Mutations in this region of the human -107/+54 promoter prevented DNA-protein interaction and lowered promoter activity. These results support the hypothesis that the GGAGGCCC-box in the human alpha 2(1) procollagen promoter has a regulatory function and that there exists a species-specific difference in transcription factor binding and regulation of the gene.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1521-6543
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
57
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
363-70
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:16036621-Amino Acid Motifs,
pubmed-meshheading:16036621-Animals,
pubmed-meshheading:16036621-Base Sequence,
pubmed-meshheading:16036621-Cell Nucleus,
pubmed-meshheading:16036621-Cells, Cultured,
pubmed-meshheading:16036621-Collagen Type I,
pubmed-meshheading:16036621-DNA,
pubmed-meshheading:16036621-DNA Methylation,
pubmed-meshheading:16036621-Fibroblasts,
pubmed-meshheading:16036621-Humans,
pubmed-meshheading:16036621-Mice,
pubmed-meshheading:16036621-Molecular Sequence Data,
pubmed-meshheading:16036621-Mutation,
pubmed-meshheading:16036621-Procollagen,
pubmed-meshheading:16036621-Promoter Regions, Genetic,
pubmed-meshheading:16036621-Protein Binding,
pubmed-meshheading:16036621-Species Specificity,
pubmed-meshheading:16036621-Transcription, Genetic,
pubmed-meshheading:16036621-Transfection
|
| pubmed:articleTitle |
Species-specific regulation of the alpha-2(I) procollagen gene by proximal promoter elements.
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| pubmed:affiliation |
Division of Medical Biochemistry, Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, South Africa.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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