Linked Life Data

16034121

Source:http://linkedlifedata.com/resource/pubmed/id/16034121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-7-21
pubmed:abstractText
To study Ag presentation in the female reproductive tract, DO11.10 TCR transgenic mice specific for the class II MHC-restricted OVA(323-339) peptide and non-transgenic BALB/c mice were used. We report here that freshly isolated uterine epithelial cells, uterine stromal, and vaginal APCs present OVA and OVA(323-339) peptide to naive- and memory T cells, which is reduced when cells are incubated with Abs to CD80 and 86. To determine whether polarized primary epithelial cells present Ags, uterine epithelial cells were cultured on cell inserts in either the upright or inverted position. After reaching confluence, as indicated by high transepithelial resistance (>2000 ohms/well), Ag presentation by epithelial cells incubated with memory T cells and OVA(323-339) peptide placed on the basolateral surface (inverted) was 2- to 3-fold greater than that seen with epithelial cells in contact with T cells and peptide on the apical surface (upright). In contrast, whereas freshly isolated epithelial cells process OVA, polarized epithelial cells did not. When epithelial cells grown upright on inserts were incubated with T cells and OVA(323-339) peptide, coculture with either hepatocyte growth factor or conditioned stromal medium increased epithelial cell Ag presentation (approximately 90% higher than controls). These studies indicate that uterine stromal cells produce a soluble factor(s) in addition to a hepatocyte growth factor, which regulates epithelial cell Ag presentation. Overall, these results demonstrate that polarized epithelial cells are able to present Ags and suggest that uterine stromal cells communicate with epithelial cells via a soluble factor(s) to regulate Ag presentation in the uterus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1795-804
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16034121-Animals, pubmed-meshheading:16034121-Antigen Presentation, pubmed-meshheading:16034121-CD4-Positive T-Lymphocytes, pubmed-meshheading:16034121-Cell Line, pubmed-meshheading:16034121-Cell Membrane, pubmed-meshheading:16034121-Cell Polarity, pubmed-meshheading:16034121-Cells, Cultured, pubmed-meshheading:16034121-Culture Media, Conditioned, pubmed-meshheading:16034121-Electric Impedance, pubmed-meshheading:16034121-Epithelial Cells, pubmed-meshheading:16034121-Female, pubmed-meshheading:16034121-G0 Phase, pubmed-meshheading:16034121-Hepatocyte Growth Factor, pubmed-meshheading:16034121-Histocompatibility Antigens Class II, pubmed-meshheading:16034121-Immunologic Memory, pubmed-meshheading:16034121-Mice, pubmed-meshheading:16034121-Mice, Inbred BALB C, pubmed-meshheading:16034121-Mice, Transgenic, pubmed-meshheading:16034121-Ovalbumin, pubmed-meshheading:16034121-Peptide Fragments, pubmed-meshheading:16034121-Stromal Cells, pubmed-meshheading:16034121-Uterus, pubmed-meshheading:16034121-Vagina
pubmed:year
2005
pubmed:articleTitle
Polarized uterine epithelial cells preferentially present antigen at the basolateral surface: role of stromal cells in regulating class II-mediated epithelial cell antigen presentation.
pubmed:affiliation
Department of Physiology, Dartmouth Medical School, Lebanon, NH 03756-0001, USA. Charles.R.Wira@Dartmouth.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural