Linked Life Data

16025536

Source:http://linkedlifedata.com/resource/pubmed/id/16025536

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-10-24
pubmed:abstractText
In this study, we consider the effect of carbon dioxide (CO(2)) on the intracellular and extracellular pH of a saline solution of a test-microorganisms Bacillus subtilis. The cytoplasmatic pH was determined by means of a flow cytometry with the fluorescent probe 5(and 6-)-carboxyfluorescein ester (cFSE). The physiological suspension of cells with the addition of the probe was first exposed to high pressure CO(2) for 5 min at different temperatures. The flow cytometry analysis indicated an intracellular depletion inside the cell caused by the action of CO(2), down to 3, the depletion being dependent on inactivation ratio. In addition, the extracellular pH was determined theoretically by means of the statistical associated fluid theory equation of state (SAFT EOS): it was demonstrated that CO(2) under pressure dissolves into liquid phase and acidifies the medium down to 3 at 80 bar and 303.15K. The results show a strong influence between extracellular and intracellular pH, and lead to the conclusion that a strong reduction of the pH homeostasis of the cell can be claimed as one of the most probable cause of inactivation of CO(2) pasteurization.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3592
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
447-51
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Determination of extracellular and intracellular pH of Bacillus subtilis suspension under CO2 treatment.
pubmed:affiliation
Department of Material Engineering and Industrial Technologies, University of Trento, via Mesiano 77, Trento, Italy. sara.spilimbergo@ing.unitn.it
pubmed:publicationType
Journal Article