Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-7-12
pubmed:databankReference
pubmed:abstractText
The regulated oscillation of protein expression is an essential mechanism of cell cycle control. The SCF class of E3 ubiquitin ligases is involved in this process by targeting cell cycle regulatory proteins for degradation by the proteasome, with the F-box subunit of the SCF specifically recruiting a given substrate to the SCF core. Here we identify NIPA (nuclear interaction partner of ALK) as a human F-box-containing protein that defines an SCF-type E3 ligase (SCF(NIPA)) controlling mitotic entry. Assembly of this SCF complex is regulated by cell-cycle-dependent phosphorylation of NIPA, which restricts substrate ubiquitination activity to interphase. We show nuclear cyclin B1 to be a substrate of SCF(NIPA). Inactivation of NIPA by RNAi results in nuclear accumulation of cyclin B1 in interphase, activation of cyclin B1-Cdk1 kinase activity, and premature mitotic entry. Thus, SCF(NIPA)-based ubiquitination may regulate S-phase completion and mitotic entry in the mammalian cell cycle.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0092-8674
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-57
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16009132-Adaptor Proteins, Signal Transducing, pubmed-meshheading:16009132-Animals, pubmed-meshheading:16009132-COS Cells, pubmed-meshheading:16009132-Cell Cycle, pubmed-meshheading:16009132-Cell Cycle Proteins, pubmed-meshheading:16009132-Cell Line, pubmed-meshheading:16009132-Cercopithecus aethiops, pubmed-meshheading:16009132-Cyclin B, pubmed-meshheading:16009132-Cyclin B1, pubmed-meshheading:16009132-Gene Silencing, pubmed-meshheading:16009132-HeLa Cells, pubmed-meshheading:16009132-Humans, pubmed-meshheading:16009132-Mice, pubmed-meshheading:16009132-Mitosis, pubmed-meshheading:16009132-Molecular Sequence Data, pubmed-meshheading:16009132-NIH 3T3 Cells, pubmed-meshheading:16009132-Nuclear Proteins, pubmed-meshheading:16009132-Phosphorylation, pubmed-meshheading:16009132-RNA, Small Interfering, pubmed-meshheading:16009132-SKP Cullin F-Box Protein Ligases
pubmed:year
2005
pubmed:articleTitle
NIPA defines an SCF-type mammalian E3 ligase that regulates mitotic entry.
pubmed:affiliation
Department of Internal Medicine III, Technical University of Munich, 81675 Munich, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural