rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-7-9
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10714,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10715,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10915,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10916,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10917,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10918,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10919,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D10920,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S37521,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S37523,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X60006
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pubmed:abstractText |
The rat hemopexin (Hx) gene was isolated and studies of its transcriptional regulation initiated. For analysis by a transient expression assay, the sequence between -2400 and +21 and sequential 5' truncates were linked to the chloramphenicol acetyltransferase (CAT) gene. HepG2 cells transfected with these CAT constructs were treated with conditioned medium of lipopolysaccharide stimulated human monocytes, interleukin-1 (IL-1) or interleukin-6 (IL-6). The activities of putative regulatory regions joined to the SV40 promoter indicated that the flanking region of the rat Hx gene from -209 to -104 contains three functional regions designated proximal regulatory regions; PRR-I (-209 to -173), -II (-178 to -158) and -III (-154 to -104). We found that PRR-II contains a different class of IL-6 responsive element (RE) from that reported for the human Hx gene, and that PRR-I and PRR-III participate in the basal expression of rat Hx in HepG2 cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
420-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1599480-Acute-Phase Reaction,
pubmed-meshheading:1599480-Amino Acid Sequence,
pubmed-meshheading:1599480-Animals,
pubmed-meshheading:1599480-Base Sequence,
pubmed-meshheading:1599480-Cloning, Molecular,
pubmed-meshheading:1599480-DNA Mutational Analysis,
pubmed-meshheading:1599480-Gene Expression Regulation,
pubmed-meshheading:1599480-Hemopexin,
pubmed-meshheading:1599480-Interleukin-1,
pubmed-meshheading:1599480-Interleukin-6,
pubmed-meshheading:1599480-Molecular Sequence Data,
pubmed-meshheading:1599480-Promoter Regions, Genetic,
pubmed-meshheading:1599480-Rats,
pubmed-meshheading:1599480-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1599480-Restriction Mapping,
pubmed-meshheading:1599480-Sequence Homology, Nucleic Acid
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pubmed:year |
1992
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pubmed:articleTitle |
Identification of an interleukin-6 responsive element and characterization of the proximal promoter region of the rat hemopexin gene.
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pubmed:affiliation |
Department of Pediatrics, Cornell University Medical College, New York, New York 10021.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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