Linked Life Data

15986864

Source:http://linkedlifedata.com/resource/pubmed/id/15986864

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2005-6-30
pubmed:abstractText
Label claims were evaluated for five probiotic products. Specific oligonucleotide primers were designed for 11 species from the Bifidobacterium, Lactobacillus, and Streptococcus genera. Polymerase chain reaction, gel electrophoresis, and amplicon excision with DNA sequencing were performed: Sequence analysis and DNA homology comparisons followed. Bifidobacterium bifidum was not detected in two of the five samples by PCR analysis. Also, Lactobacillus species were found in two of the five product samples for which the species was not listed as an ingredient. We conclude that (1) lack of B. bifidum in two probiotic products may be attributed to different preparation standards among probiotic manufacturers, and (2) identificaition of additional Lactobacillus species may represent contamination of the samples related to the fact that manufacturers utilize shared equipment to produce all probiotics and PCR is a highly sensitive technique.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0163-2116
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1113-7
pubmed:dateRevised
2006-4-13
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Evaluation of five probiotic products for label claims by DNA extraction and polymerase chain reaction analysis.
pubmed:affiliation
University of Kansas Medical Center, Program in Integrative Medicine, Kansas City, Kansas 66160, USA. jdrisko@kumc.edu
pubmed:publicationType
Journal Article