Source:http://linkedlifedata.com/resource/pubmed/id/15977236
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2005-6-30
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| pubmed:abstractText |
Phylogenetic analysis and evaluation of drug-resistance were carried out upon 59 plasma samples from 58 treatment-naïve HIV-1 infected patients from Mozambique, enrolled in a free antiviral-therapy protocol in the frame of Drug-Resource-Enhancement against AIDS and Malnutrition (DREAM) programme. Sequencing of the first 1,300 bases of the pol-gene shows that all virus strains cluster within clade C, with the exception of a single patient carrying a G-subtype virus. Relevant mutations in the reverse transcriptase (RT) are rare: 118A/I/L/G (four patients), 179E/D/I (three patients), 333E/D (two patients), 101R, and 210F (one patient each). In Protease (PR), V82I (10.3%) is the only relevant mutation, while natural polymorphisms/secondary mutations are found, some at very high frequency: 20R (25.9%), 36I (91.4%), 36L (8.6%), 60E (31.0%), 63P (29.3%), and 93L (96.6%). Among them, mutations with a frequency >25% were further investigated to assess their covariation pattern with PI resistance associated mutations. The pattern of covariation observed for K20R and D60E (but not L63P and M36I) was different between C and B subtype isolates from PR-inhibitor-treated patients. The sequences were also analyzed to calculate the ratio of non-synonymous to synonymous substitution. The ratio for PR and RT was 0.116 and 0.093, respectively, suggesting a greater conservation in RT than PR in both subtypes B and C HIV strains. Taken together, the results demonstrate a consistent clade-homogeneity of viral strains circulating in Mozambique, and the very limited presence, in drug-naïve patients, of mutations associated with resistance to RT-inhibitors. The high frequency of secondary mutations/polymorphisms in HIV-PR deserves further studies to evaluate its relevance in clinical settings.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
|
| pubmed:issn |
0146-6615
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| pubmed:author |
pubmed-author:BellocchiMaria ConcettaMC,
pubmed-author:CeffaSusannaS,
pubmed-author:CoelhoElisabethE,
pubmed-author:D'ArrigoRobertaR,
pubmed-author:Emberti-GialloretiLeonardoL,
pubmed-author:ErbaFulvioF,
pubmed-author:ForbiciFedericaF,
pubmed-author:GoriCaterinaC,
pubmed-author:MarazziMaria CristinaMC,
pubmed-author:PalombiLeonardoL,
pubmed-author:PernoCarlo-FedericoCF,
pubmed-author:SilbersteinFrancesca CeccheriniFC,
pubmed-author:SvicherValentinaV
|
| pubmed:copyrightInfo |
(c) 2005 Wiley-Liss, Inc.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
76
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
452-8
|
| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15977236-Amino Acid Substitution,
pubmed-meshheading:15977236-Anti-HIV Agents,
pubmed-meshheading:15977236-Drug Resistance, Viral,
pubmed-meshheading:15977236-Genes, pol,
pubmed-meshheading:15977236-Genotype,
pubmed-meshheading:15977236-HIV Infections,
pubmed-meshheading:15977236-HIV Protease,
pubmed-meshheading:15977236-HIV Reverse Transcriptase,
pubmed-meshheading:15977236-HIV-1,
pubmed-meshheading:15977236-Humans,
pubmed-meshheading:15977236-Mozambique,
pubmed-meshheading:15977236-Mutation,
pubmed-meshheading:15977236-Phylogeny
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: results from the DREAM programme.
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| pubmed:affiliation |
INMI L, Spallanzani, Rome, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|