Linked Life Data

15969013

Source:http://linkedlifedata.com/resource/pubmed/id/15969013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-6-22
pubmed:abstractText
The hematopoietic system of the mouse arises from extraembryonic mesoderm that migrate through primitive streak to the presumptive yolk sac at day 7.0 of gestation. However, the mechanisms regulating mesoderm commitment to hematopoietic lineages remain poorly understood. Previous studies demonstrated that the development kinetics and growth factor responsiveness of hematopoietic precursors derived from embryonic stem cells (ES cells) is similar to that found in the yolk sac, indicating that the onset of hematopoiesis within the embryoid bodies (EBs) parallels that found in the embryo. Furthermore, in vitro differentiation of ES cells to hematopoietic cells is valuable for establishment of therapeutic clone against a variety of hematological disorders. Despite the identification of multipotential hematopoietic progenitors in EBs, a subset of more primitive progenitors, identical to the high proliferative potential colony-forming cells (HPP-CFC) derived from human and murine hematopoietic tissues, have not been clearly identified regarding particular their replating potential in vitro. HPP-CFC is among the most primitive hematopoietic multipotent precursors cultured in vitro. In this study, our aim was to investigate the in vitro and in vivo hematopoietic capacity of HPP-CFC within the day 12 EBs, rather than the expansion of more committed progenitors. In this study the HPP-CFC could be detected within EBs differentiated for 5 to 14 days of murine ES cells, but the development dynamics of the HPP-CFC differed greatly among distinct serum lots. Qualitatively HPP-CFC is capable of forming secondary colonies. As to our expectation the ES cells-derived HPP-CFC demonstrated similar regeneration capacity to those from yolk sac, giving rise to secondary granulocyte, erythrocyte, macrophage and mast cells, however largely differed from the counterparts of adult bone marrow. In addition, by RT-PCR ES cells-derived HPP-CFC were found to express transcription factors associated closely with stem cell proliferation including SCL, GATA-2 and AML1 as well as various receptors of hematopoietic growth factors such as c-kit, GM-CSF receptor and interleukin 3 receptor et al. Finally, in order to understand the in vivo hematopoietic capacity of the ES cells-derived HPP-CFC, spleen colony-forming unit (CFU-S) assay was performed. Nevertheless, typical CFU-S was not observed after transplantation of the day 12 EB cells or HPP-CFC colonies into lethally irradiated adult murine. In conclusion the HPP-CFC differentiated from murine ES cells displayed robust hematopoietic activity in vitro, however their in vivo reconstitution ability was not detected. The difference between in vitro and in vivo hematopoietic activities of ES cells-derived primitive hematopoietic precursors deserves further investigation.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-6
pubmed:meshHeading
pubmed-meshheading:15969013-Animals, pubmed-meshheading:15969013-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:15969013-Cell Differentiation, pubmed-meshheading:15969013-Colony-Forming Units Assay, pubmed-meshheading:15969013-Core Binding Factor Alpha 2 Subunit, pubmed-meshheading:15969013-Embryonic Stem Cells, pubmed-meshheading:15969013-GATA2 Transcription Factor, pubmed-meshheading:15969013-Hematopoietic Stem Cells, pubmed-meshheading:15969013-Humans, pubmed-meshheading:15969013-Mice, pubmed-meshheading:15969013-Proto-Oncogene Proteins, pubmed-meshheading:15969013-Proto-Oncogene Proteins c-kit, pubmed-meshheading:15969013-Receptors, Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:15969013-Receptors, Interleukin-3, pubmed-meshheading:15969013-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2003
pubmed:articleTitle
[The investigation of hematopoietic capacity of HPP-CFC derived from murine embryonic stem cells in vitro and in vivo].
pubmed:affiliation
Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't