Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1992-7-9
|
| pubmed:abstractText |
Interleukin-4 (IL-4) regulates the growth of B cells. When combined with colony-stimulating factors (CSFs) and selected cytokines, IL-4 has a synergistic effect on the clonal growth of bone marrow cells. Recently, we have shown that IL-1 alpha and lipopolysaccharide induce expression of the granulocyte-macrophage CSF (GM-CSF) gene in murine B-cell lines. In the present study, we show that IL-4 inhibits the production of GM-CSF in the IL-1 alpha-stimulated murine B-cell line M12.4.1. IL-4 did not change the transcription rate of the GM-CSF gene, and caused only a slight decrease in cytoplasmic GM-CSF messenger RNA (mRNA) half-life in cells treated with IL-1 alpha. PCR analysis of nuclear RNA with probes specific for GM-CSF intron sequences suggests that IL-1 alpha enhances accumulation of nuclear precursor RNA and that decreased GM-CSF expression after IL-4 treatment is mainly due to intranuclear destabilization of the primary transcript. Under the same experimental conditions, IL-4 did not affect expression of the IL-4 receptor mRNA and did increase the mRNA concentration of the low-affinity receptor for IgE (Fc epsilon RII). These data suggest that the suppressive effect of IL-4 is specific for GM-CSF mRNA expression, and thus provide evidence for an additional role of IL-4 in the regulation of GM-CSF expression in B cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
79
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3188-95
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1596564-Animals,
pubmed-meshheading:1596564-B-Lymphocytes,
pubmed-meshheading:1596564-Base Sequence,
pubmed-meshheading:1596564-Blotting, Northern,
pubmed-meshheading:1596564-Cell Nucleus,
pubmed-meshheading:1596564-Gene Expression Regulation,
pubmed-meshheading:1596564-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1596564-Half-Life,
pubmed-meshheading:1596564-Interleukin-1,
pubmed-meshheading:1596564-Interleukin-4,
pubmed-meshheading:1596564-Lymphoma, B-Cell,
pubmed-meshheading:1596564-Mice,
pubmed-meshheading:1596564-Molecular Sequence Data,
pubmed-meshheading:1596564-RNA, Messenger,
pubmed-meshheading:1596564-RNA Precursors,
pubmed-meshheading:1596564-Recombinant Proteins,
pubmed-meshheading:1596564-Transcription, Genetic,
pubmed-meshheading:1596564-Tumor Cells, Cultured
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Interleukin-4 inhibits interleukin-1 alpha-induced granulocyte-macrophage colony-stimulating factor gene expression in a murine B-lymphocyte cell line via downregulation of RNA precursor.
|
| pubmed:affiliation |
Division of Cytokine Biology, Food and Drug Administration, Bethesda, MD 20892.
|
| pubmed:publicationType |
Journal Article
|