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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2005-6-9
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pubmed:abstractText |
Synapse formation requires interactions between pre- and postsynaptic cells to establish the connection of a presynaptic nerve terminal with the neurotransmitter receptor-rich postsynaptic apparatus. At developing vertebrate neuromuscular junctions, acetylcholine receptor (AChR) clusters of nascent postsynaptic apparatus are not apposed by presynaptic nerve terminals. Two opposing activities subsequently promote the formation of synapses: positive signals stabilize the innervated AChR clusters, whereas negative signals disperse those that are not innervated. Although the nerve-derived protein agrin has been suggested to be a positive signal, the negative signals remain elusive. Here, we show that cyclin-dependent kinase 5 (Cdk5) is activated by ACh agonists and is required for the ACh agonist-induced dispersion of the AChR clusters that have not been stabilized by agrin. Genetic elimination of Cdk5 or blocking ACh production prevents the dispersion of AChR clusters in agrin mutants. Therefore, we propose that ACh negatively regulates neuromuscular synapse formation through a Cdk5-dependent mechanism.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Agrin,
http://linkedlifedata.com/resource/pubmed/chemical/Bungarotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Carbocyanines,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Choline O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cholinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 5,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Hb9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscarine,
http://linkedlifedata.com/resource/pubmed/chemical/N,N'-diethylthiacarbocyanine iodide,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptophysin,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/roscovitine
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
569-79
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15944126-Acetylcholine,
pubmed-meshheading:15944126-Agrin,
pubmed-meshheading:15944126-Animals,
pubmed-meshheading:15944126-Blotting, Western,
pubmed-meshheading:15944126-Bungarotoxins,
pubmed-meshheading:15944126-Carbachol,
pubmed-meshheading:15944126-Carbocyanines,
pubmed-meshheading:15944126-Cell Line,
pubmed-meshheading:15944126-Choline O-Acetyltransferase,
pubmed-meshheading:15944126-Cholinergic Agonists,
pubmed-meshheading:15944126-Cyclin-Dependent Kinase 5,
pubmed-meshheading:15944126-Cyclin-Dependent Kinases,
pubmed-meshheading:15944126-Diaphragm,
pubmed-meshheading:15944126-Drug Interactions,
pubmed-meshheading:15944126-Embryo, Mammalian,
pubmed-meshheading:15944126-Female,
pubmed-meshheading:15944126-Homeodomain Proteins,
pubmed-meshheading:15944126-Immunohistochemistry,
pubmed-meshheading:15944126-Immunoprecipitation,
pubmed-meshheading:15944126-In Situ Hybridization,
pubmed-meshheading:15944126-Mice,
pubmed-meshheading:15944126-Mice, Knockout,
pubmed-meshheading:15944126-Muscarine,
pubmed-meshheading:15944126-Muscle, Smooth,
pubmed-meshheading:15944126-Muscle Fibers, Skeletal,
pubmed-meshheading:15944126-Neural Inhibition,
pubmed-meshheading:15944126-Neuromuscular Junction,
pubmed-meshheading:15944126-Pregnancy,
pubmed-meshheading:15944126-Protein Kinase Inhibitors,
pubmed-meshheading:15944126-Purines,
pubmed-meshheading:15944126-Receptor Aggregation,
pubmed-meshheading:15944126-Receptors, Cholinergic,
pubmed-meshheading:15944126-Synaptophysin,
pubmed-meshheading:15944126-Time Factors,
pubmed-meshheading:15944126-Transcription Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Neurotransmitter acetylcholine negatively regulates neuromuscular synapse formation by a Cdk5-dependent mechanism.
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pubmed:affiliation |
The Salk Institute, La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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