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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1992-7-2
|
| pubmed:abstractText |
To identify the target genes modulated by the myb gene product (Myb), a co-transfection assay with a Myb expression plasmid was performed. Both c-Myb and B-Myb, another member of the myb gene family, trans-activated the human c-myc promoter. DNAase I footprint analysis using the bacterially expressed c-Myb, identified multiple c-Myb binding sites in the c-myc promoter region. Deletion analysis of the c-myc promoter suggested that some number of Myb binding sites, not a specific Myb binding site, is important for the c-Myb-induced trans-activation of the c-myc promoter. Using the c-myc-chloramphenicol acetyltransferase (CAT) construct as a reporter in a co-transfection assay, the domains of c-Myb required for trans-activation were examined. The functional domains of c-Myb identified using the c-myc promoter were almost the same as those identified previously with the artificial target gene containing Myb binding sites, but unlike the case with the artificial target gene the N-terminal half of the previously identified negative regulatory domains and the C-terminal 136 amino acids were required for the maximal trans-activation of the c-myc promoter. These results indicate that there are some differences in the regulation of Myb-dependent trans-activation in different target genes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:geneSymbol |
B-myb,
c-myb,
c-myc
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1233-40
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1594249-Animals,
pubmed-meshheading:1594249-Base Sequence,
pubmed-meshheading:1594249-Cell Line,
pubmed-meshheading:1594249-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1594249-Chromosome Deletion,
pubmed-meshheading:1594249-DNA-Binding Proteins,
pubmed-meshheading:1594249-Genes, myc,
pubmed-meshheading:1594249-Humans,
pubmed-meshheading:1594249-Molecular Sequence Data,
pubmed-meshheading:1594249-Oligodeoxyribonucleotides,
pubmed-meshheading:1594249-Oncogenes,
pubmed-meshheading:1594249-Promoter Regions, Genetic,
pubmed-meshheading:1594249-Proto-Oncogene Proteins,
pubmed-meshheading:1594249-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:1594249-Recombinant Fusion Proteins,
pubmed-meshheading:1594249-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1594249-Transcription, Genetic,
pubmed-meshheading:1594249-Transcriptional Activation,
pubmed-meshheading:1594249-Transfection
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Transcriptional activation of the c-myc gene by the c-myb and B-myb gene products.
|
| pubmed:affiliation |
Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|