Linked Life Data

15934200

Source:http://linkedlifedata.com/resource/pubmed/id/15934200

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-6-5
pubmed:abstractText
We investigated the spatial distribution of the induction of the phosphorylated form of the histone protein H2AX (gamma-H2AX), known to be activated by DSBs. Following irradiation of human fibroblast cells with 600 MeV/nucleon silicon and 600 MeV/nucleon iron ions we observed the formation of gamma-H2AX aggregates in the shape of streaks stretching over several micrometers in an x/y plane. Polyethylene shielding was used to achieve a Bragg curve distribution with beam geometry parallel to the monolayer of cells. We present data that highlights the formation of immunofluorescent gamma-H2AX tracks showing the ion trajectories across the Bragg peak of irradiated human fibroblast cells. Qualitative analyses of these distributions indicated potentially increased clustering of DNA damage before the Bragg peak, enhanced gamma-H2AX distribution at the peak, and provided visual evidence of high-linear energy transfer particle traversal of cells beyond the Bragg peak in agreement with one-dimensional transport approximations. Spatial assessment of gamma-H2AX fluorescence may provide direct insights into DNA damage across the Bragg curve for high charge and energy ions including the biological consequences of shielding and possible contributors to bystander effects.
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0273-1177
pubmed:author
pubmed:copyrightInfo
c2005 Published by Elsevier Ltd on behalf of COSPAR.
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
236-42
pubmed:dateRevised
2007-4-16
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
High LET-induced H2AX phosphorylation around the Bragg curve.
pubmed:affiliation
NASA Johnson Space Center, Houston, TX 77058, USA.
pubmed:publicationType
Journal Article