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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1992-7-1
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pubmed:abstractText |
Amylose-defective mutants were selected after UV mutagenesis of Chlamydomonas reinhardtii cells. Two recessive nuclear alleles of the ST-2 gene led to the disappearance not only of amylose but also of a fraction of the amylopectin. Granule-bound starch synthase activities were markedly reduced in strains carrying either st-2-1 or st-2-2, as is the case for amylose-deficient (waxy) endosperm mutants of higher plants. The main 76-kDa protein associated with the starch granule was either missing or greatly diminished in both mutants, while st-2-1-carrying strains displayed a novel 56-kDa major protein. Methylation and nuclear magnetic resonance analysis of wild-type algal storage polysaccharide revealed a structure identical to that of higher-plant starch, while amylose-defective mutants retained a modified amylopectin fraction. We thus propose that the waxy gene product conditions not only the synthesis of amylose from endosperm storage tissue in higher-plant amyloplasts but also that of amylose and a fraction of amylopectin in all starch-accumulating plastids. The nature of the ST-2 (waxy) gene product with respect to the granule-bound starch synthase activities is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-13760681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-16664339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-16665043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-17245994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-17249118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-2005870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-2216792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-2700539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-2970062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-4042112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-4824506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-6052439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-6057640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1592815-6313224
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3612-20
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1592815-Alleles,
pubmed-meshheading:1592815-Amylopectin,
pubmed-meshheading:1592815-Amylose,
pubmed-meshheading:1592815-Animals,
pubmed-meshheading:1592815-Chlamydomonas reinhardtii,
pubmed-meshheading:1592815-Chloroplasts,
pubmed-meshheading:1592815-Kinetics,
pubmed-meshheading:1592815-Mutation,
pubmed-meshheading:1592815-Selection, Genetic,
pubmed-meshheading:1592815-Starch,
pubmed-meshheading:1592815-Starch Synthase
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pubmed:year |
1992
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pubmed:articleTitle |
Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin.
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pubmed:affiliation |
Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille Flandres-Artois 59655, Villeneuve d'Ascq, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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