| pubmed-article:15922966 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0001483 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1332717 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1706438 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0085358 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1517806 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1413244 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C2698600 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0439859 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0439677 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:15922966 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:15922966 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:15922966 | pubmed:dateCreated | 2005-5-30 | lld:pubmed |
| pubmed-article:15922966 | pubmed:abstractText | Acute myeloid leukemia (AML) cells can be differentiated into dendritic cells (DCs) using appropriate combinations of cytokines but generation of autologous antileukemic cytotoxic T cells using leukemic DCs remains difficult. Transduction by adenoviral vectors has been reported to induce efficient maturation of monocyte-derived DCs but AML cells are generally resistant to adenoviral gene transfer. In this study we tested the effects of adenoviral TNF-alpha gene transfer on maturation of AML cells using the fiber-modified AdTNF.F(pK7) adenovirus. All samples expressed high and sustained levels of TNF-alpha following transduction. AdTNF.F(pK7) induced significantly greater maturation of AML cells into antigen-presenting cells (APC) than did recombinant TNF-alpha or control adenoviral vector. Maturation of leukemic cells into APCs was mediated at least partially via a PI3K/mTOR pathway, as the inhibitors LY294002, wortmannin, and rapamycin inhibited the maturation effect induced by the AdTNF.F(pK7) adenovirus. In addition, CD8+ T cells expanded with AdTNF.F(pK7)-transduced AML cells showed greater expansion and specific CD8+ CTL activity against autologous AML cells than T cells expanded by other means. Thus, fiber-modified adenoviral vectors encoding TNF-alpha are able to maturate AML cells into APCs with high efficacy and reproducibility, providing a useful tool to generate efficiently specific CD8+ CTLs against leukemic disease. | lld:pubmed |
| pubmed-article:15922966 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15922966 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15922966 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15922966 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15922966 | pubmed:issn | 1525-0016 | lld:pubmed |
| pubmed-article:15922966 | pubmed:author | pubmed-author:QuesnelBrunoB | lld:pubmed |
| pubmed-article:15922966 | pubmed:author | pubmed-author:WickhamThomas... | lld:pubmed |
| pubmed-article:15922966 | pubmed:author | pubmed-author:SaudemontAuro... | lld:pubmed |
| pubmed-article:15922966 | pubmed:author | pubmed-author:CormSelimS | lld:pubmed |
| pubmed-article:15922966 | pubmed:author | pubmed-author:HetuinDominiq... | lld:pubmed |
| pubmed-article:15922966 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15922966 | pubmed:volume | 11 | lld:pubmed |
| pubmed-article:15922966 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15922966 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15922966 | pubmed:pagination | 950-9 | lld:pubmed |
| pubmed-article:15922966 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:15922966 | pubmed:meshHeading | pubmed-meshheading:15922966... | lld:pubmed |
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| pubmed-article:15922966 | pubmed:meshHeading | pubmed-meshheading:15922966... | lld:pubmed |
| pubmed-article:15922966 | pubmed:meshHeading | pubmed-meshheading:15922966... | lld:pubmed |
| pubmed-article:15922966 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15922966 | pubmed:articleTitle | Induction of leukemia-specific CD8+ cytotoxic T cells with autologous myeloid leukemic cells maturated with a fiber-modified adenovirus encoding TNF-alpha. | lld:pubmed |
| pubmed-article:15922966 | pubmed:affiliation | Unité INSERM 524, Institut de Recherche sur le Cancer de Lille, 59037 Lille, France. | lld:pubmed |
| pubmed-article:15922966 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15922966 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
