Linked Life Data

15919722

Source:http://linkedlifedata.com/resource/pubmed/id/15919722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2005-8-26
pubmed:abstractText
Androgen receptor (AR) is important in male sexual differentiation and testicular function. Here, we demonstrate the regulation of AR expression and its transactivation by the basic helix-loop-helix (bHLH) transcription factor Pod-1, the expression of which in postnatal testis reciprocally coincides with the expression of AR. Pod-1 represses the promoter activity of AR, possibly through its E-box. An AR promoter region of 169 bp, which harbors one canonical E-box, is sufficient for the Pod-1-repression and bound by purified Pod-1 proteins. Pod-1 also suppresses the transactivation of AR. Transient transfection analyses of mammalian cells show that Pod-1 represses AR transactivation in a dose-dependent manner. Furthermore, yeast two-hybrid, glutathione-S-transferase-pull-down, and co-immunoprecipitation analyses reveal that Pod-1 directly associates with AR through its N-terminal region and through the DNA binding-hinge domain of AR. Interestingly, Pod-1 recruits histone deacetylase (HDAC)-1 to inhibit both promoter activity and transactivation of AR. Overexpression of HDAC1 further inhibits the Pod-1-mediated repressions and Pod-1 directly interacts with HDAC1. Furthermore, chromatin immunoprecipitation assay reveals that HDAC1 is recruited with Pod-1 to the endogenous AR promoter and the androgen-regulated Pem promoter. Taken together, these results suggest that Pod-1, which controls AR transcription and function, may play an important role in the development and function of the testis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2245-57
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15919722-Animals, pubmed-meshheading:15919722-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:15919722-Cells, Cultured, pubmed-meshheading:15919722-Chromatin Immunoprecipitation, pubmed-meshheading:15919722-Down-Regulation, pubmed-meshheading:15919722-E-Box Elements, pubmed-meshheading:15919722-Gene Expression Regulation, pubmed-meshheading:15919722-Histone Deacetylase 1, pubmed-meshheading:15919722-Histone Deacetylases, pubmed-meshheading:15919722-Homeodomain Proteins, pubmed-meshheading:15919722-Humans, pubmed-meshheading:15919722-Male, pubmed-meshheading:15919722-Mice, pubmed-meshheading:15919722-Promoter Regions, Genetic, pubmed-meshheading:15919722-Receptors, Androgen, pubmed-meshheading:15919722-Sequence Deletion, pubmed-meshheading:15919722-Testis, pubmed-meshheading:15919722-Transcription Factors, pubmed-meshheading:15919722-Transcriptional Activation, pubmed-meshheading:15919722-Two-Hybrid System Techniques
pubmed:year
2005
pubmed:articleTitle
Modulation of the expression and transactivation of androgen receptor by the basic helix-loop-helix transcription factor Pod-1 through recruitment of histone deacetylase 1.
pubmed:affiliation
Hormone Research Center, School of Biological Sciences and Research, Chonnam National University, Gwangju 500-757, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't