| pubmed-article:15908172 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0870134 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0162371 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0229601 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0040952 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:15908172 | lifeskim:mentions | umls-concept:C0681828 | lld:lifeskim |
| pubmed-article:15908172 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15908172 | pubmed:dateCreated | 2005-11-8 | lld:pubmed |
| pubmed-article:15908172 | pubmed:abstractText | Previous studies have revealed that hematological disorders associated with trichothecenes intoxication in humans could result from hematopoiesis inhibition. The most frequent and potent trichothecene mycotoxins are T-2 toxin and deoxynivalenol (DON), respectively. Apoptosis induction by these two toxins was investigated in vitro on human hematopoietic progenitors (CD34+ cells). Hoechst coloration, DNA fragmentation and annexin-V/PI labeling in flow cytometry showed that T-2 toxin, in contrast to DON, induced apoptosis in CD34+ cells. T-2 toxin effect was dose- and time-dependent with a significant increase of apoptotic cells as early as 3h after incubation at 10(-7) M and a maximum reached at 12 h. This observation evidenced the high sensitivity of hematopoietic progenitors to T-2 toxin. The inhibition of T-2 toxin-induced apoptosis by a pan-caspase inhibitor (Z-VAD-fmk) suggested the involvement of caspases. The proportional increase of caspase-3 specific activity (DEVDase) with T-2 toxin concentration confirmed its role in the process. After incubation of CD34+ cells with T-2 toxin, in conditions that induced apoptosis, clonal expansion of granulo-monocytes, erythrocytes and megakaryocytes precursors was dose-dependently inhibited. The hematological effects observed in T-2 toxin mycotoxicosis could then be assigned to hematopoiesis inhibition by apoptosis. Different mechanisms that need to be further elucidated are involved in DON myelotoxicity. | lld:pubmed |
| pubmed-article:15908172 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15908172 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15908172 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15908172 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15908172 | pubmed:issn | 0887-2333 | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:AuffretMM | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:ArnoldFF | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:SibirilYY | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:BatinaPP | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:Le DréanGG | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:Parent-Massin... | lld:pubmed |
| pubmed-article:15908172 | pubmed:author | pubmed-author:ArzurDD | lld:pubmed |
| pubmed-article:15908172 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15908172 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:15908172 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15908172 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15908172 | pubmed:pagination | 1015-24 | lld:pubmed |
| pubmed-article:15908172 | pubmed:dateRevised | 2009-4-10 | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
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| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:meshHeading | pubmed-meshheading:15908172... | lld:pubmed |
| pubmed-article:15908172 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15908172 | pubmed:articleTitle | Myelotoxicity of trichothecenes and apoptosis: an in vitro study on human cord blood CD34+ hematopoietic progenitor. | lld:pubmed |
| pubmed-article:15908172 | pubmed:affiliation | Laboratoire de Toxicologie Alimentaire, EA 3880, Université de Bretagne Occidentale, Technopôle Brest-Iroise, 29280 Plouzané, France. gwenola.ledrean@univ-brest.fr | lld:pubmed |
| pubmed-article:15908172 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15908172 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15908172 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15908172 | lld:pubmed |
