Linked Life Data

15904680

Source:http://linkedlifedata.com/resource/pubmed/id/15904680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-5-20
pubmed:abstractText
Three puffer fishes, Fugu rubripes, collected from the Bohai Sea of China were examined for tetrodotoxin-producing microorganisms. An actinomycete isolated from the ovaries of the puffer fishes was found to produce tetrodotoxin. After being cultured at 28 degrees C for 7 days, cells were harvested by centrifuge and disrupted by ultrasonication. The toxin was purified from the cell lyzate by ultrafiltration, active charcoal column, Bio-gel-p2 and ion exchange column chromatography. Mouse neuroblastoma cell culture, thin-layer chromatography, fluorimetric spectrophotometry, UV-spectrophotometry and electrospray ionization mass spectrometry, together with mouse bioassay demonstrated that the isolated strain produced tetrodotoxin and related toxin during cultivation. Based on morphological, physiological, biochemical characteristics and 16S rDNA alignment, this strain was identified as Nocardiopsis dassonvillei. Our findings suggested that N. dassonvillei in the ovaries was closely related to the toxification of the puffer fish.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
851-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
A new tetrodotoxin-producing actinomycete, Nocardiopsis dassonvillei, isolated from the ovaries of puffer fish Fugu rubripes.
pubmed:affiliation
Department of Biological Science and Biotechnology, Tsinghua University, Beijing, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't