Source:http://linkedlifedata.com/resource/pubmed/id/15902977
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-5-20
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| pubmed:abstractText |
Non-steroidal anti-inflammatories (NSAIDs) are analgesic, antipyretic, and, as their name implies, anti-inflammatory drugs, which are widely used for the treatment of a variety of human and veterinary disease conditions in which control of pain and inflammation is desired. Acetaminophen (ACE) is a common over-the-counter analgesic. Detection of a variety of widely used NSAIDs and ACE in fluid and tissue samples is an important diagnostic tool. A sensitive and selective analytical method has been developed for simultaneous screening of 12 NSAIDs and ACE by liquid chromatography-mass spectrometry with an atmospheric pressure chemical ionization interface set to operate in the negative ion mode of MS. Following sample preparation, all analytes were separated on a C18-reversed-phase column with a gradient elution of acetonitrile and acetic acid. Full-scan mass spectral fragmentation profiles were established for each analyte and individual extracted ion chromatograms were used for quantitation. Linearity of detection was observed over the 0.05-25.0 microg/mL range of standard concentrations. The instrument limits of detection (LOD), based on an individual analyte quantitation ions, fell between 0.05 and 1.0 microg/mL for all compounds. The matrix LODs were determined to be 0.05 microg/mL for phenylbutazone (m/z 307); 0.1 microg/mL for indomethacin (m/z 312), flunixin (m/z 295), and piroxicam (m/z 330); 0.5 microg/mL for ACE (m/z 150), diclofenac (m/z 250), ketoprofen (m/z 209), and mefenamic acid (m/z 240); 1.0 microg/mL for oxyphenbutazone (m/z 323); 5.0 microg/mL for ibuprofen (m/z 205), salicylic acid (m/z 137), and tolmetin (m/z 212); and 10 microg/mL for naproxen (m/z 185).
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0146-4760
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
95-104
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15902977-Acetaminophen,
pubmed-meshheading:15902977-Animals,
pubmed-meshheading:15902977-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:15902977-Cattle,
pubmed-meshheading:15902977-Chromatography, Liquid,
pubmed-meshheading:15902977-Mass Spectrometry,
pubmed-meshheading:15902977-Reproducibility of Results,
pubmed-meshheading:15902977-Serum
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Multi-residue determination of anti-inflammatory analgesics in sera by liquid chromatography--mass spectrometry.
|
| pubmed:affiliation |
University of Pennsylvania, School of Veterinary Medicine, Department of Pathobiology, New Bolton Center, Toxicology, 382 West Street Road, Kennett Square, Pennsylvania 19348, USA. irudik@vet.upenn.edu
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| pubmed:publicationType |
Journal Article
|