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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2005-5-23
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pubmed:abstractText |
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2926-32
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15890343-Animals,
pubmed-meshheading:15890343-Base Sequence,
pubmed-meshheading:15890343-DNA Primers,
pubmed-meshheading:15890343-Dimerization,
pubmed-meshheading:15890343-Homeodomain Proteins,
pubmed-meshheading:15890343-Mice,
pubmed-meshheading:15890343-Nerve Tissue Proteins,
pubmed-meshheading:15890343-Otx Transcription Factors,
pubmed-meshheading:15890343-Repressor Proteins,
pubmed-meshheading:15890343-Transcription, Genetic
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pubmed:year |
2005
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pubmed:articleTitle |
Functional analysis of transcriptional repressor Otx3/Dmbx1.
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pubmed:affiliation |
Division of Cellular and Molecular Medicine, Kobe University, Graduate School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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