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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2005-5-18
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pubmed:abstractText |
The mutation sufY204 mediates suppression of a +1 frameshift mutation in the histidine operon of Salmonella enterica serovar Typhimurium and synthesis of two novel modified nucleosides in tRNA. The sufY204 mutation, which results in an amino-acid substitution in a protein, is, surprisingly, dominant over its wild-type allele and thus it is a "gain of function" mutation. One of the new nucleosides is 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U34) modified by addition of a C(10)H(17) side chain of unknown structure. Increased amounts of both nucleosides in tRNA are correlated to gene dosage of the sufY204 allele, to an increased efficiency of frameshift suppression, and to a decreased amount of the wobble nucleoside mnm(5)s(2)U34 in tRNA. Purified tRNA(Gln)(cmnm(5)s(2)UUG) in the mutant strain contains a modified nucleoside similar to the novel nucleosides and the level of aminoacylation of tRNA(Gln)(cmnm(5)s(2)UUG) was reduced to 26% compared to that found in the wild type (86%). The results are discussed in relation to the mechanism of reading frame maintenance and the evolution of modified nucleosides in tRNA.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1842-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15861125-Amino Acid Substitution,
pubmed-meshheading:15861125-Frameshift Mutation,
pubmed-meshheading:15861125-Genes, Suppressor,
pubmed-meshheading:15861125-Lac Operon,
pubmed-meshheading:15861125-Nucleosides,
pubmed-meshheading:15861125-Operon,
pubmed-meshheading:15861125-RNA, Transfer,
pubmed-meshheading:15861125-Salmonella typhimurium,
pubmed-meshheading:15861125-Selenium Compounds,
pubmed-meshheading:15861125-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:15861125-Transfer RNA Aminoacylation
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pubmed:year |
2005
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pubmed:articleTitle |
A "gain of function" mutation in a protein mediates production of novel modified nucleosides.
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pubmed:affiliation |
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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