Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1992-6-16
pubmed:abstractText
The Jun protein is a transcription factor of the AP-1 complex, and it is concentrated in the cell nucleus. While the cellular Jun protein is transported into the nucleus in a cell-cycle-independent fashion, the oncogenic viral version of the protein translocates into the nucleus most rapidly during the G2 phase of the cell cycle and only slowly during G1 and S phases. This cell cycle dependence of nuclear transport has been mapped to the cysteine to serine mutation in the carboxyl-terminal portion of viral Jun. We have identified a complex nuclear translocation signal located in the basic region of viral Jun. This signal has the sequence ASKSRKRKL. A peptide of this sequence synthesized in vitro and conjugated to IgG can mediate cell-cycle-dependent translocation of the microinjected conjugate from the cytoplasm into the nucleus. The nuclear translocation signal has two functional domains. The pentapeptide RKRKL is sufficient as a cell-cycle-independent nuclear address. The entire signal is needed for cell-cycle-dependent nuclear translocation. The amino-terminal tetrapeptide contains the cysteine to serine substitution responsible for cell cycle dependence. Deletion analysis of the Jun protein suggests that the nuclear translocation signal identified in the basic region is required for nuclear translocation of Jun and may be the only such signal in the Jun molecule.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-1309952, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-1652372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-1712670, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-1848177, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-1991319, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2004116, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2118682, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2123464, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2157987, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2167175, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2448875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2494630, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2577867, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2825349, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-2855501, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-3015419, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-3041020, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-3142689, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-3900074, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-6330534, http://linkedlifedata.com/resource/pubmed/commentcorrection/1584763-6725403
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
89
pubmed:geneSymbol
c-jun, jun, v-jun
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4290-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:1584763-Amino Acid Sequence, pubmed-meshheading:1584763-Animals, pubmed-meshheading:1584763-Base Sequence, pubmed-meshheading:1584763-Cell Cycle, pubmed-meshheading:1584763-Cell Nucleus, pubmed-meshheading:1584763-Cells, Cultured, pubmed-meshheading:1584763-Chick Embryo, pubmed-meshheading:1584763-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1584763-Fluorescent Antibody Technique, pubmed-meshheading:1584763-G2 Phase, pubmed-meshheading:1584763-Genes, jun, pubmed-meshheading:1584763-Immunoglobulin G, pubmed-meshheading:1584763-Kinetics, pubmed-meshheading:1584763-Molecular Sequence Data, pubmed-meshheading:1584763-Mutagenesis, Site-Directed, pubmed-meshheading:1584763-Oligodeoxyribonucleotides, pubmed-meshheading:1584763-Oncogene Protein p65(gag-jun), pubmed-meshheading:1584763-Peptides, pubmed-meshheading:1584763-Protein Processing, Post-Translational, pubmed-meshheading:1584763-Proto-Oncogene Proteins c-jun, pubmed-meshheading:1584763-Rabbits
pubmed:year
1992
pubmed:articleTitle
Nuclear translocation of viral Jun but not of cellular Jun is cell cycle dependent.
pubmed:affiliation
Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033-1054.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't