Linked Life Data

15811997

Source:http://linkedlifedata.com/resource/pubmed/id/15811997

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-4-6
pubmed:abstractText
Lactobacillus casei ASCC 292 was grown in the presence of six prebiotics, namely, sorbitol, mannitol, maltodextrin, high-amylose maize, fructooligosaccharide (FOS), and inulin, in order to determine the combination of probiotic and prebiotics that would remove the highest level of cholesterol. A first-order model showed that the combination of L. casei ASCC 292, FOS, and maltodextrin was the most efficient for the removal of cholesterol, and the optimum experimental region was developed by using the steepest ascent. This led to the middle points of probiotic (1.70% [wt/vol]), FOS (4.80% [wt/vol]), and maltodextrin (6.80% [wt/vol]) for the development of a central composite design for optimization. Perturbation plot, response surface, and coefficient estimates showed that all three factors had significant quadratic effects on cholesterol removal, with FOS showing the most conspicuous quadratic change. A second-order polynomial regression model estimated that the optimum condition of the factors for cholesterol removal by L. casei ASCC 292 is 1.71% (wt/vol) probiotic, 4.95% (wt/vol) FOS, and 6.62% (wt/vol) maltodextrin. Validation experiments showed that the predicted optimum conditions were more efficient than the high and low levels of the factors and the center points. A response surface method proved reliable for developing the model, optimizing factors, and analyzing interaction effects. Analyses of growth, substrate utilization, growth yield, mean doubling time, and short-chain fatty acid (SCFA) production by the use of quadratic models indicated that cholesterol removal was growth associated. The concentration of L. casei ASCC 292 had the most significant quadratic effect on all responses studied, except for substrate utilization and SCFA production, which were significantly (P < 0.05) influenced by the interactions between the probiotic and both prebiotics, indicating that they were closely associated with the uptake of prebiotics.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-10067658, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-10232648, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-10395607, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-10721912, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-10801928, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-11157351, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-11451676, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-12676703, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-1533273, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-15591367, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-7782892, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-8526490, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-8872712, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-9526834, http://linkedlifedata.com/resource/pubmed/commentcorrection/15811997-9706221
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1745-53
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Optimization of cholesterol removal by probiotics in the presence of prebiotics by using a response surface method.
pubmed:affiliation
School of Molecular Sciences, Victoria University, Melbourne, Victoria, Australia.
pubmed:publicationType
Journal Article