Linked Life Data

15808528

Source:http://linkedlifedata.com/resource/pubmed/id/15808528

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-4-5
pubmed:abstractText
This study focused on whether bone marrow would differentiate tolerogenic dendritic cells (DCs) by comparing interleukin-3 (IL-3) DCs (induced by IL-3+IL-4) with GM-CSF DCs (induced by GM-CSF+IL-4, generating myeloid DCs). Mouse bone marrow progenitors were induced in vitro to differentiate into DCs by exposure to IL-3. The morphology and yields of IL-3 DCs and GM-CSF DCs were compared by scanning electron microscopy and laser scanning confocal microscopy. Immunohistochemistry and flow cytometer (FCM) were used to identify immune phenotypes. The results showed that IL-3 DCs and GM-CSF DCs both expressed the specific marker DEC-205 with about 80% and 70% purity, respectively. IL-3 DCs had similar morphology as plasma cells, but a different immune phenotype, namely, CD80(-), CD86(-), CD14(-), and MHC class II(+). IL-3 DCs remained in the costimulatory molecule-deficient state when TNF-alpha was supplemented into the culture. We therefore propose that IL-3 induces a high purity and yield of DCs from bone marrow. Since IL-3 DCs possess a tolerogenic phenotype (MHC class II(+), B7(-)), they may induce alloantigen-specific hyporesponsiveness in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0041-1345
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
The characteristics of tolerogenic plasmacytoid dendritic cells stimulated with interleukin-3.
pubmed:affiliation
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, P.R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't