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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0021760,
umls-concept:C0026249,
umls-concept:C0031727,
umls-concept:C0033634,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0667931,
umls-concept:C1366876,
umls-concept:C1833235,
umls-concept:C1879547,
umls-concept:C1948023,
umls-concept:C2911684
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pubmed:issue |
6
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pubmed:dateCreated |
2005-3-16
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pubmed:abstractText |
Adenosine binds to a class of G-protein coupled receptors, which are further distinguished as A(1), A(2a), A(2b) and A(3) adenosine receptors. As we have shown earlier, the stable adenosine analogue NECA (N6-(R)-phenylisopropyladenosine) stimulates IL-6 expression in the human astrocytoma cell line U373 MG via the A(2b) receptor. The mechanism by which NECA promotes astrocytic IL-6 expression has not been identified. By using various inhibitors of signal transduction, we found that p38 mitogen-activated protein kinases (MAPK) activation (inhibitor SB202190), but not extracellular signal-regulated kinase (ERK) (PD98059) and c-jun N-terminal kinase (JNK)(SP600125), is essential in the NECA-induced signalling cascade that leads to the increase in IL-6 synthesis in U373 MG cells. Results obtained with protein kinase C (PKC) inhibitors that have different substrate specificities, indicated that the PKC delta and epsilon isoforms are also involved in adenosine receptor A(2b) dependent upregulation of IL-6 expression. This is supported by the fact that NECA induced the activation of PKC delta and epsilon in U373 MG cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine A2 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCE protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-delta,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-epsilon,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Adenosine A2B,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0197-0186
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-12
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15769552-Adenosine,
pubmed-meshheading:15769552-Adenosine A2 Receptor Agonists,
pubmed-meshheading:15769552-Astrocytoma,
pubmed-meshheading:15769552-Cell Line, Tumor,
pubmed-meshheading:15769552-Enzyme Inhibitors,
pubmed-meshheading:15769552-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:15769552-Humans,
pubmed-meshheading:15769552-Interleukin-6,
pubmed-meshheading:15769552-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:15769552-Protein Kinase C,
pubmed-meshheading:15769552-Protein Kinase C-delta,
pubmed-meshheading:15769552-Protein Kinase C-epsilon,
pubmed-meshheading:15769552-RNA, Messenger,
pubmed-meshheading:15769552-Receptor, Adenosine A2B,
pubmed-meshheading:15769552-Signal Transduction,
pubmed-meshheading:15769552-Up-Regulation,
pubmed-meshheading:15769552-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2005
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pubmed:articleTitle |
IL-6 expression induced by adenosine A2b receptor stimulation in U373 MG cells depends on p38 mitogen activated kinase and protein kinase C.
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pubmed:affiliation |
University of Freiburg Medical School, Department of Psychiatry and Psychotherapy, Neurochemistry Research Group, Hauptstrasse 5, D-79104 Freiburg, Germany. bernd.fiebich@klinikum.uni-freiburg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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