Source:http://linkedlifedata.com/resource/pubmed/id/15737205
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-3-1
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| pubmed:abstractText |
Detection of micrometastases in the regional tumor-draining lymph nodes is critical for staging and prognosis in melanoma patients. We applied a quantitative multiple-marker RT-PCR assay to improve the detection of occult melanoma cells in the sentinel lymph node (SLN). From 139 patients with primary cutaneous melanoma who underwent sentinel lymphadenectomy, a total of 235 SLN were assessed for Melan-A and tyrosinase expression by real-time quantitative RT-PCR. Twenty-three patients (17%) were positive by histopathology and expressed messenger RNA of one or two markers. Of the patients with histopathologically negative SLN 39 (28%) were reclassified by positive RT-PCR. Patients were examined for tumor recurrences during a median follow-up period of 29 mo. Tumor recurrences were demonstrated in eight patients (35%) with histopathologically positive SLN, in four patients (10%) with submicroscopic tumor cells detected exclusively by real-time RT-PCR, and in none of the patients negative by both methods. The differences in recurrence rates were statistically significant (p=0.01). These data indicate that real-time quantitative RT-PCR for the detection of minimal residual melanoma in SLN improves the prediction of disease-free survival.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Monophenol Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0022-202X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
124
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
633-7
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:15737205-Antigens, Neoplasm,
pubmed-meshheading:15737205-Disease-Free Survival,
pubmed-meshheading:15737205-Follow-Up Studies,
pubmed-meshheading:15737205-Genetic Testing,
pubmed-meshheading:15737205-Humans,
pubmed-meshheading:15737205-Lymph Node Excision,
pubmed-meshheading:15737205-Lymphatic Metastasis,
pubmed-meshheading:15737205-MART-1 Antigen,
pubmed-meshheading:15737205-Melanoma,
pubmed-meshheading:15737205-Monophenol Monooxygenase,
pubmed-meshheading:15737205-Neoplasm, Residual,
pubmed-meshheading:15737205-Neoplasm Proteins,
pubmed-meshheading:15737205-Neoplasm Recurrence, Local,
pubmed-meshheading:15737205-Predictive Value of Tests,
pubmed-meshheading:15737205-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15737205-Risk Factors,
pubmed-meshheading:15737205-Sentinel Lymph Node Biopsy,
pubmed-meshheading:15737205-Skin Neoplasms
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| pubmed:year |
2005
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| pubmed:articleTitle |
Quantification of melanoma micrometastases in sentinel lymph nodes using real-time RT-PCR.
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| pubmed:affiliation |
Institute of Immunology, University of Heidelberg, Heidelberg, Germany. Thomas.Geise@urz.uni-heidelberg.de
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| pubmed:publicationType |
Journal Article,
Clinical Trial
|